154 COCAINE. 



COCAINE. 



1. Make two muscle preparations. Place them in glass capsules, and obtain nor- 

 mal curves with both the slow and fast drums. Then fill one capsule with Ringer's 

 solution, and the other with Ringer's containing 0.1% solution Cocaine Hydrochloride. 

 Take records every half minute, letting every third record be on the fast drum. Com- 

 pare results for indications. 



2. Make two nerve-muscle preparations. Place both in watch-glasses containing 

 Ringer's solution, but have the nerves exposed on filter paper liberally wet one 

 with Ringer's, the other with 2% Cocaine solution. Make tests every 20 seconds of 

 nerve conductivity, using weak single-shock break stimuli. 



3. Expose heart of pithed frog, and obtain normal tracings. Continuing trac- 

 ings, flood heart with 2% solution Cocaine. 



4. Obtain normal tracings of heart of pithed frog. Inject i mil 0.3% solution 

 Cocaine into lymph sac of thigh. 



5. Into a rabbit's eye place I drop of a 2% solution Cocaine. Essay an explana- 

 tion of ensuing phenomena. 



6. Using an etherized cat, obtain the usual mechanical registrations; also tem- 

 perature. Into the jugular vein run a warm 0.5% solution Cocaine (in Locke's solu- 

 tion) at the rate of i mil every 2 minutes. Continue until animal dies. 



7. Place some 2% solution on the lip and on the side of the tongue. After 3 to 

 5 minutes, test parts for sensitivity. 



8. Using a small dog, inject subcutaneously 2.5 mg. x kg. Cocaine. Follow tem- 

 perature variations for 2 hours. 



9. In the minor surgery clinic, and in the clinics of operative ophthalmology and 

 rhinology, secure data concerning the utility of Cocaine in obtunding sensation. 



10. Consult the literature for the relative merits of the various Cocaine substitutes. 



EXPERIMENT No. i. 

 Kymograph records of Cocaine action on frog muscle: 



EXPERIMENT No. 2. 

 Report of Cocaine action on nerve conductivity: 



