-32- 



e. Czapeck's agar cultures to obtain general types of growth 

 of strains 1, 2, 3, 5 and 12. 



This formula was choeen because it was a complete nutrient medium, 

 because it could be readily duplicated by other workers, and because due 

 to its lack of color, end because of its transparent qualities it was 

 considered a desirable medium to use in photographing the cultures. 



Poured plates were made in the usual way and were inoculated with 

 the five strains, 1, 2, 3, 4 and 12, August 16, 1917. The photographs 

 of these five strains were taken August 28, 1918 or twelve days after 

 the plates were inoculated. Plates 21-25. These are typical photographs 

 of the different cultures. 



Strain 1 produced spores abundantly. In obtaining the color of the 

 pustules the petri dish was placed over the color chart, the lid of the 

 dish having first been renoved. The spore pustules of this? strain were 

 very close in color to pale olive-buff (R). The general outline and 

 appearance of the culture may be seen from the photograph of this strain. 

 Plate 21. 



Strain 2 produced a compact, somewhat moist looking culture that was 

 barely raised above the surface of the agur, and to the unaided ey* 

 there seeded to be no spores present. An examination of material froa 

 the culture under the microscope, revealed some spore-like pieces of 

 mycelium, but no typical spores. Plate 22. 



Strain 3, called by Dr. ^esterdijk Sclerotinia fructigena 

 produced spores abundantly and was so similar to etrain 1 that the two 

 could not be distinguished. Plate 23. 



The same was true of strain 4, which to begin with had the felty- 

 looking, cartridge Buff pustules. Plate 24. 



