-34- 



with the five strains used for the Czapeck'e agar cultures, 

 1, 2, 3, 4 and 12. This was done on August 17, 1917. Five check 

 punctures were made, onebelow each inoculation oint, and these remained 

 unchanged until overgrown by the fungus from above. 1he photograph 

 was taken August 27th, 1917, or ten days after inoculation. Plate 26. 

 In it the five letters, A, B, C. D, fi represent the strains 1, 2, 3, 

 12 and 4 respectively. 



A, C ; E or strains 1, 3, 4 again could not be distinguished. They 

 produced spores abundantly, especially A and . The pustules were a 

 little lighter than drab (C). B, or strain 2, grew so slowly that it 

 was soon surrounded by A and C. Strain 2 produced a circular brown 

 decayed spot, with a crack in the epiderads of the apple running through 

 the point of inoculation. Microscopic examination revealed the fact that 

 there t?ere typical conidia present in the crack. 



D, or strain 12, produced its most characteristic pustules, 

 immediately around the letter. These were typically compact, felty- 

 locking, and were cartridge buff(R) In color. 



3hus again there were shat eeemed to be three distinct strains. 



1 he inoculation of this appls termin&tfld the experimental work. 



