Leukaemia of the Fowl. 11 



5. Mast cell (g) : About same size and shape. Nucleus round or 

 oval, very pale blue. Cytoplasm abundant, colorless, mostly to one 

 side of nucleus with purple granules, some scattered over nucleus. 



Differential Count, 300 Cells. 



Per cent. 



Polymorphonuclear with eosinophilic rods 29.6 



Polymorphonuclear with eosinophilic granules 4.3 



Lymphocyte 42.3 



Large mononuclear cell 19.4 



Mast cell 2.2 



Unclassified cells 2.2 



100.0 

 AUTOPSY PROTOCOL. 



The following findings would correspond to those in a fowl of the 

 average size and weight (1760 gm.) of the animals injected. 



Subcutaneous Fat Abundant (Fig. 4). Thick muscle pad covering 

 the skeleton (Fig. 4). Inner surface of skin slightly yellow. 



Cervical Lymph-glands * (Figs. 3, a and b). Sometimes difficult to 

 find. Present as two chains, each consisting of six to ten glands, 

 one on each side of neck, lying upon the internal jugular vein, buried 

 in fat, and extending from the middle of neck to base of heart. They 

 are delicate, elliptical, flat, lobulated bodies, averaging 1 x .5 x .2 cm. 

 The parenchymatous lobules are pink and separated from each other 

 by narrow septa of fat. No other lymph-glands were found in the 

 entire body. 



Thyroids and Parathyroids. Two of each gland. The thyroid, a 

 dark red, translucent, spindle-shaped body, 1 x .4 x .4 cm., with 

 just visible colloid-filled acini, separated by delicate gray septa. The 

 parathyroid is attached to its lower pole. This is a small bluish- 

 white sphere, 2 mm. in diameter. Thus, in pairs, they are found, one 

 pair on each side of the body, just above the heart. 



Peritoneal Cavity (Fig. 4). An omen turn composed entirely of fat, 

 1 cm. in thickness, takes its origin from the anterior surface and lower 

 margin of the gizzard, extending over coils of intestine, etc., holding 



* Kon * considers these structures the thymus. He 4 and Ellermann M " 

 state that the fowl has no lymph-glands. Soshestrenski 5 asserts their 

 existence by stating that in his case they were not enlarged. 



