u6 



Shiro Tashiro 



6. NERVE OF VERTEBRATE : Nerves of frog, dog, mouse, squiteague (cynoscion 



Regalis), and skate. (Both Raid Ocallata and Raia Erinecia.) 



7. NERVE or WARM-BLOODED ANIMALS: Those of dog, mouse and rabbits. 



8. NERVE OF COLD-BLOODED ANIMALS: Frog, squiteague (cynoscion Regalis) 



and skate. (Both Raia Ocallata and Raia Erinecia.) 



From this I have concluded that isolated nerves of all animals 

 give off CO 2 . It remains, now, to consider whether this CO2 is the 

 product of normal respiratory activity or due to disintegration of the 

 dead tissue. 



IS THE C0 2 GIVEN OFF PRODUCED BY LIVING PROCESSES? 



Comparison of Dead and Living Nerves. In the first place, it 

 was thought that if C02 was due to normal metabolism of a living 

 nerve, its production should be diminished when the nerve was killed. 

 The following result (Table III) is self explanatory. 



TABLE III 



COMPARISON BETWEEN NORMAL AND KILLED (BY STEAM) NERVES OF SPIDER CRAB 

 123 4567 



Comparison of Anaesthetized and Non-Anaesthetized Nerves. 

 It is naturally feared, however, that the killing experiment itself may 

 not prove that C0 2 production is necessarily due to the living mechan- 

 ism, for high temperature may drive off C0 2 produced already by the 

 process of tissue disintegration, just as the C0 2 diffused out from a 

 wet thread saturated with the gas, the rate of diffusion being a func- 

 tion of temperature. Thus anaesthesia was tried, although we should 



