61 



Sftanination of canned peas to distinguish soaked goods Continued. 

 SOAKED PEAS. 



REPORT ON THE SEPARATION OF MEAT PROTEIDS. 



w i : i ; 1 1 M i : , Associate Referee. 



lMirin-4 the past year no ON. jMT.it iv- work has been asked for on the separation of 

 but considerable work has been dune at the Missouri experiment sta- 

 tion, in conni'ctioii with tin- u'eneral meat investigations, in the examination of cold 

 \vaN-r i-\traei> of meat from composite samples of various wholesale cuts of steers of 

 different ages and of different d-^rees of fatness. 



In tin- preparation ! cold water extracts the general plan as proposed by Grindley 

 and Kmmett " was followed with slight modifications. 



With lean samples exactly 100 grams and with fat samples 150 grams were taken; 

 in each case being distributed through eighteen beakers in about equal portions. 

 Thi-.-anijili- in <M' h h.-.ik.T was thoroughly mixed with 3 to 5 cc of cold neutral nitrogen- 

 ii-iilltil \\.iifi and thru with 50 cc of the flame water. The mixture was allowed 

 nd about thirty ininut'-s, with fn-qiu-nt agitation, and then poured through pre- 

 viously \\.-it.-d tiltrrs. Each time the residue which was poured on the filter was 

 returned to the beaker. TO th.- n-~idue in the beaker 25 cc of water were added and 

 the i te thoroughly stirred and again filtered. This washing was continued 



till _'_' iter were u-rd; then the whole residue was transferred to the filter and 



washed tui< e \\ith 10 to 15 cc of water each time. 



The filtrates were eombined, the lla.-ks ringed, and the total volume made up to 



5,000 cc. This cold water extract was carefully mixed without undue agitation and 



filtered through a dry tiller just before the aliquot portions were taken for analysis. 



1 n all tiltrations the" funnel was made to touch the sides of the flask or beaker, so as to 



limit the amount of spontaneous coagulation.) 



Triplicate samples were taken for analysis as follows: 



a, 6, c. 100 cc for total soluble nitrogen representing 2 grams of lean and 3 grams 

 of fat sample. 



rf, e, /. 100 cc for total solids and ash representing 2 grams of lean and 3 grams of 

 fat sample. * * * 



m, n, o. 200 cc for coagulable nitrogen representing 4 grams of lean and 6 grams of 

 fat sample. 



J. Amer. ('hem. Soc., 1905, 27: 661. 



