62 



p> ?> r. 200 cc for total albumoee nitrogen; filtrate from TO, n, o, representing 4 

 grams 'lean and 6 grams of fat sample. 



s, t, u. 200 cc for total amido acid nitrogen representing 4 grams of lean and 6 grams 



Samples a, 6, c were transferred to 500 cc nitrogen flasks for the direct determination 

 of nitrogen by the Kjeldahl-Gunning method. 



Samples d, e,fwere evaporated to dryness on the water bath, then dned to constant 

 weight in air bath at 103, and finally ashed at a dull red heat. 



Samples TKL, n, o were treated with a slight exces^ of moist magnesium carbonate, a 

 evaporated to about 30 cc, filtered and washed with hot water to which a little moist 

 magnesium carbonate had been added. The precipitate and filter were transferred 

 to 600 cc nitrogen flasks and the coagulum adhering to the sides of the beakers was 

 removed with hot sulphuric acid and transferred to the corresponding flask. The 

 nitrogen was determined in the usual manner. 



The filtrates from TO, n, o, (p, q, r) were concentrated to about 10 cc in small beakers 

 and acidified with 1 cc of 50 per cent sulphuric acid, diluted to 30 cc and to each 50 

 grams of pure crystallized zinc sulphate were added. The mixture was then heated 

 upon the water bath until the complete solution of the zinc sulphate took place. If too 

 much zinc sulphate crystallized out upon cooling a little water was added, care being 

 taken to have only a slight excess above saturation. The contents of the beakers were 

 filtered through filters previously wet with a saturated solution of zinc sulphate 

 slightly acidified with sulphuric acid. After the filtrate had completely drained 

 through, the beaker and filter were washed three times with the saturated zinc sulphate 

 solution, allowing the washing to drain completely before adding the next washing. 

 The filter and precipitate were transferred to nitrogen flasks and each beaker washed 

 with water and sulphuric acid, the washings being rinsed into the corresponding flask. 

 If care has been used in avoiding an excess of zinc sulphate crystals there will be no 

 trouble with bumping during the digestion for the determination of the albumose 

 nitrogen. 



Samples , t, u were treated as for coagulable nitrogen. The filtrates from the coag- 

 ulum, not to exceed 30 cc, were rinsed into 100 cc graduated flasks, 15 grams of sodium 

 chlorid were added and dissolved by warming gently. The flasks were placed in the 

 ice box until cooled to 15 C. A, 24 per cent solution of tannic acid was made up, fil- 

 tered, and cooled in the ice box. When both solutions were cooled, 30 cc of the tannic 

 acid solution were added to each 100 cc flask, which was then filled to the mark with 

 cold water; the contents of the flask were thoroughly mixed and allowed to remain 

 in the ice box over night. The following morning they were filtered rapidly and 50 cc 

 of the filtrate transferred to nitrogen flasks for the determination of the amido acid 

 nitrogen. With all of the determinations blanks were made to correct for the nitrogen 

 in the reagents. From these data the nitrogen present as peptone nitrogen was cal- 

 culated. 



The main purpose in the examination of the water extracts of the fresh meats has 

 been to see if age of animal or condition of fatness has any influence upon the amount 

 of water-soluble material, or upon its composition, also to what extent there is a varia- 

 tion in different parts of the animal. To this end the samples have been handled as 

 nearly as possible in the same manner and with the same treatment after slaughtering. 

 So far eight animals have been slaughtered and analyzed, but the data are still insuffi- 

 cient to admit of any general conclusions, and the present paper is to be regarded only 

 as a report of progress. A tabulation of a few of the results is appended, selecting those 

 for the round and rump, the rib and loin cuts of the first four animals slaughtered. 



a Prepared by precipitating magnesium chlorid with sodium carbonate, heating, 

 filtering, and washing until no chlorids remained in the filtrate. 



