179 APPENDIX A 



Petri dish was placed in an incubator and kept at 28 30C. 

 At 6 p.m. on the same day there were plain indications of 

 colonies forming but they were too small to allow a separation 

 count. 



May 5th, 10 a.m. 7 colonies of Bacteria and 4 of Fungi 



showing. 

 ,, 6th, 10 a.m. 16 colonies of Bacteria and 5 of Fungi 



showing. 

 ,, Tth, 10 a.m. 23 colonies of Bacteria and 6 of Fungi 



showing. 

 ,, 8th, 10 a.m. 30 colonies of Bacteria and 6 of Fungi 



showing. 



That is, in 96 hours, 30 colonies of Bacteria and 6 colonies 

 of Fungi were observed. 



The fungi were examined and identified as follows : 



2 colonies of Saccharomyces sp. 



2 , , , , Penicillium glaucum. 



1 colony ,, Aspergillus niger. 



1 ,, ,, Cladosporium herbarum. 



The bacteria were removed in the ordinary manner and 

 were sub-cultured, plated out and identified as follows : 



Micrococcus urece 2 colonies. 



Bacillus subtilis 7 , , 



Bacillus coli commune ... 11 ,, 



Sarcina lutea 2 ,, 



Stained by Gram 3 ,, 



Not stained by Gram ... 5 ,, 



Experiment No. 2. 



A fly was caught at 11.30 a.m. on May 4th out of doors 

 on Central Hill, Norwood, London, and was allowed to walk 

 over nutrient agar-agar at 12 o'clock noon. 



