APPENDIX A 180 



May 4th, 6 p.m. Colonies were plainly forming. 

 ,, 5th, 10 a.m. 13 colonies of Bacteria and 6 colonies of 



Fungi. 

 ,, 6th, 10 a.m. 21 colonies of Bacteria and 7 colonies of 



Fungi. 

 ,, 7th, 10 a.m. 39 colonies of Bacteria and 7 colonies of 



Fungi. 

 ,, 8th, 10 a.m. 46 colonies of Bacteria and 7 colonies of 



Fungi. 



That is, in 94 hours, 46 colonies of Bacteria and 7 colonies 

 of Fungi were obtained from this fly No. 2. The fungi 

 were identified as follows : 



2 colonies of Macrosporium sp. 



3 ,, ,, Penicillium glaucum. 



1 colony ,, Cladosporium herbarum. 

 1 ,, ,, Fusarum roseum. 



The bacteria after being sub-cultured and plated out were 

 identified as : 



Bacillus tumescens 18 colonies. 



Micrococcus pyogenes aureus 9 ,, 



Sarcina lutea 2 ,, 



Sarcina ventriculi 1 colony. 



Bacillus amylobacter 4 colonies. 



Acid fast bacillus .. 1 colony. 



Stained by gram 4 colonies. 



Not stained by gram 7 ,, 



Experiment No. 3. 



This experiment was perhaps the most interesting of the 

 three as the fly was captured at 10.30 a.m. on May 4th on 

 a dust bin (Norwood, London), a situation in which flies are 

 frequently found. It was allowed to walk over the surface 

 of nutrient agar-agar. 



