12 DEPARTMENT OF AORICULTURE 



In both of these anin.als the walls of the bladder were found at the autopniy to be 

 one centimetre in thickness. This increase of the calcium salts in the urine seems 

 to be responsible for the great irritation following urination manifested by switch- 

 ing of the tail and eversion of the clitoris. In two of the cases which we have had 

 here the urine has contained a great quantity of mucin. 



In the seminal fluid of two diseased stallions collected after the covering of 

 mares, Dr. Hawden found very few spermatozoa, and even these ceased their move- 

 ments very quickly in coverslip preparations. The fluid ejected from these stal- 

 lions was plentiful in supply, and from its general appearance was considered to be 

 largely of prostatic origin. 



The vaginal mucous membrane is pale and of a leaden hue with some scars. The 

 mucous membrane is in folds and there is an (Edematous condition of the os, but 

 otherwise nothing abnormal is noted. The mucous membrane of the uterus is usually 

 of a leaden colour and oedematous. In some cases the cedematous folds were very 

 pronounced, increasing the size of the organ considerably. The ovaries were invari- 

 ably cystic, though I am not prepared to assert that this was due to the infectious 

 process under discussion. None of the mares upon which I held autopsies had recently 

 shown a profuse vaginal discharge, indicating that all were more or less chronic. 



In addition to the general lesions described above, the testicles of two stallions 

 were atrophied, in one instance the process had progressed to an almost complete 

 absence of testicular tissue. In one of these stallions ' Brucefield Prince,' (Plate 

 IV and V) there was a diffuse cedematous swelling of the sheath. No marked changes 

 were noted about the penis save a slight roughness of the mucous membrane of the 

 urethra. 



Sleepy 588. 



This mare. (bay. aged, weighing about 1.600 pounds) was quarantined in the 

 spring of 1904. condemned in May, 1905, held for experimental observation and trans- 

 ferred to the Biological Laboratory, Ottawa, in the fall of that year arriving on 

 November 22. On arrival she was in excellent physical condition and apart from the 

 evidences of the disease which were to be noted at a careful examination of the 

 mucous membrane of the vagina, she appeared to be perfectly healthy, although she 

 would at times manifest a slight knuckling of the hind fetlocks, but this manifesta- 

 tion was by no means well marked, nor would it. under ordinary conditions, admit of 

 detection.* 



She was stabled during the winter and at various times furnished material for 

 animal inoculations. As the major portion of our work was conducted with the other 

 mare coming from this infected herd (Puss 589)', I will not detail the experiments 

 conducted with material from this individual. 



In the spring of 1906 she was bred to a stallion (660) purchased for that purpose, 

 in an endeavour to obtain a recently infected animal that a suitable case for the 

 detection of the parasite might be available. This course was pursued as a careful 

 study of the literature indicated that a recently infected stallion was more likely to 

 give positive results in the detection of the parasite, than would follow repeated exam- 

 inations of material from animals in other stages of the disease. Our efforts in this 

 direction, however, were productive of no data indicating an infection. 



A detailed record of the blood examinations of this mare is to be found in connec- 

 tion with the remarks upon ' Blood Examinations in Dourine,' appended to this report. 

 A number of examinations of vaginal smears were made but the temper of the mare 

 rendered our efforts in this direction particularly difficult and dangerous. In none 

 of these vaginal smears were we able to detect evidence of parasites nor of forms 

 simulating them. 



•Prior to the transfer of this mare to Ottawa marked clinical manifestations of the disease 

 under consideration, at one time presented themselves. 



