DOURINE OR MALADIE DU COIT 17 



(Boulger, 670) did not result in impregnation. This latter mare was purposely bred 

 late in the period of oestrum that impregnation might be avoided, the desire being 

 to infect her from 589 through the stallion 650. 



This stallion has at no time presented any evidence that the covering of the 

 mare (589) resulted in an infection. 



During the breeding season of 1907 he has been used on the mares (588, 589 and 

 670), and up to the present writing, Novemb'ei^ 15, there is no clinical evidence of a 

 dourine infection in the stallion or in the mare (670). 



From the time of his purchase till August 8, 1907, he was kept at the laboratory 

 stable, having the use of the paddock for exercise. On that date, however, he was 

 taken by the agricultural division of the Experimental Farm for work where he has 

 maintained his excellent physical condition. 



By working him it was anticipated that in the event of an infection, clinical 

 manifestations would be present much earlier than would be the case with only the 

 exercise obtainable in a .small inclosure. 



A record of blood examination made can be found in connection with our 

 remarks on 'Blood Examinations in Dourine.' (Table V.) 



Manotick^ 660. 



This stallion was purchased on June 9, 1906, for the purpose of conducting 

 breeding experiments with maladie du coit. He was an aged stallion, but had f\ 

 history indicating that he had never been further than fifty miles from Ottawu. 

 He provided a cheap experimental subject for our purposes and was bred to the 

 mare (588 Sleepy) only. Services with this mare were somewhat difficult owing to 

 the stallion's small size, but we were able to effect them on June 20 and 21 and on 

 July 13. As will be seen from the tables of blood counts of this horse (Table VT.), 

 there was never a marked variation in the polynuclear elements, and but slight 

 variations in the lymphocytes, and these could be accounted for by the increased 

 number of eosinophile cell&, the presence of which in the blood was due to the para- 

 sites which were found at autopsy. The existence of this parasitism was recognized 

 from the first, but it was considerfed that it would interfere but little with our 

 dourine experiments. 



The death of the stallion occurred on October 16, 1906, as a result of the nema- 

 tode parasitic invasion, and there had never been any evidence during the entire 

 period he was under observation that he had become, infected with the trypanosoma 

 for which we were searching. 



The autopsy revealed the following lesion.s: — 



Some subcutaneous and intermuscular o?dema was noted, particvilarly at the hips, 

 withers, and in the sterr.ial regions. The oedema, however, did not exhibit the chara- 

 acteristics noted in dourine-infected animals. 



The majority of the joints of both the fore and hind limbs were opened without 

 finding any evidence of articular lesions.. The heart was enlarged and its muscle 

 was firm. The lungs were normal. The spleen was normal weighing 780 grammes. 

 The liver was normal save the presence of small nodules about the size of bird shot 

 which were found to be parasitic in their origin. The stomach contained a large 

 number of larvae of Oestms equii. The small intestine was free from parasitic inva- 

 sion. The caecum and anterior portion of the colon contained large numbers of 

 sclerostomata and there were many small tumors in the mucous membrane, the largest 

 of these being about the size of a hickory nut, containing bloody, purulent matter. 

 This bloody, purulent material proved on miscroscopic examination to consist of 

 degenerated leucocytes many of which were eosinophiles. No other parasites than those 

 above mentioned were noted in the intestinal tract. The left kidney weighed 570 

 grammes and presented evidence of cloudy swelling. The right kidney weighed 420 

 grammes, was inflamed and oedema tons. The afferent and efferent blood vessels were 



9459—2 



