18 DEPARTMENT OF AORICULTURE 



involved in a mass of diseased tissue containing a nodule about one inch in diameter 

 and this nodule was composed of very hard fibrous tissue with calcareous deposits. 

 Tliorc was also cnntninod in this mass an aneurism of the renal artery and a dilation 

 of the ureter. Nematode parasites (Sclerastoma Armahim) were found in the aneu- 

 rism and in the dilation of the ureter. The walls of each cavity were from one-half 

 to three-quarters of an inch in thickness. The bladder contained very dark coloured 

 urine in which there was much mucus. The ascitic fluid was scanty, contained a few 

 nematode worms (Filaria papilosa) and one of these was found in the scrotal sac. 



There was in none of the organs a condition simulating that commonly found 

 in cases of dourine. 



BOULGER, 670. 



This is an aged mare which was purchased on June 9, 1906, in order that a healthy 

 mare might be available for bneeding with the stallion (650) after he had covered the 

 dourined mare (589 Puss). Boulger (G70) was bred a number of times during the 

 season of 1906, but we have failed to find any evidence of her having contracted the 

 disease. During the season of 1907 she had been bred to the same stallion (650) he 

 having previously covered the mares (588 and 589). 



No evidence of an infection in this mare has presented itself, the mare being in 

 first class condition and there has never been any swelling or distortion of the external 

 genitals nor has there been anv vaffinal dischaxge or abnormal appearance of the 

 mucous membrane 



BLOOD EXAMINATIONS IN DOUEINE. 



Shortly after the receipt at the Biological Laboratory, of the mares affected with 

 dourine for experimental purposes, some blood studies were undertaken with a view 

 of determining the pathological changes which were taking place. 



"We were, from the very inception of this work greatly interested in the changes 

 which manifested themselves and have since embraced every opportunity afforded for 

 increasing our knowledge of the subject, with the result, that we are now able to 

 present some interesting data. 



In detailing this work it is first necessary to indicate the method of differentiat- 

 ing the various cells that our tables and general remarks may be more fully under- 

 stood. 



We have considered the enumeration of the blood cells under four general varie- 

 ties, one of which has been subdivided into four divisions. 

 1 Poly-morpho-nuclear Neutrophiles, 

 2. Eosinophiles, 



3 Mast cells, 



4 Total lymphocytes : 



a. Lymphocytes (large and small), 



b. Large mononuoloan. 



c. Granular mononuclear, 



d. Transitional basophiles. 



For a description of the general characteristics of the varieties mentioned, their 

 source and functions, the reader is referred to the text books dealing with the normal 

 and pathological histology of the blood. 



In addition to the cells mentioned we have also enumerated those celLs which 

 could not be classed in any of the above subdivisions but which from their peculiar 

 staining characteristics seemed to identify themselves as transitional neutrophiles. 



A mueh better eonception of the details of our classification can be obtained 

 by referring to the coloured plate (Plato VIIT.) than can be given by any descrip- 

 tion which we mav offer. 



