DO URINE OR UAL AD IE DU COIT 37 



paration of blood smears or the collection of blood for examination. This procedure 

 has in my hands greatly facilitated the search for the parasites and has now become 

 habitual with me. I have repeatedly secured preparations before and after irrigation, 

 finding the parasites only in those taken after. 



Some points of interest. — Three months elapsed after the covering by the Cly- 

 desdale stallion, (No. 35), and the first manifestation of the disease. Notable fea- 

 tures of the case are — the absence during these three months, according to the ani- 

 mal's owner, of any lesions of the genital organs and the fact that the disease instead 

 of being primarily localized to these organs, as is usual, became rapidly generalized. 



The nervous phenomena were the first noticeable manifestations of the disease, 

 by which the diagnosis was made, being confirmed 78 days later by the finding of the 

 trypanosomata. A marked sexual excitement has at all times been evident on bring- 

 ing the mare in proximity to a stallion. 



To the profuse blood-letting, which was pushed! to the physiological limit, I attri- 

 bute the disappearance of the nervous symptoms, by the removal with the blood of a 

 great amount of toxine of the disease. 



EXPERIMENTAL INOCULATIONS WITH DOURINE. 



EQUINES. 



Animal No. 26. — Two year old filly. ' The vaginal discharges of two dourined 

 mares were injected into the vagina and uterus, last injection made July 18, 1906. No. 

 positive reaction obtained.' — (Dr. S. Hadwen.) Blood inoculations. — November 21, 

 1906. Intraperitoneal inoculation of 30 c.c. of citrated blood from stallion 33 (jugular 

 blood). 



November 27. Intramusciilar inoculation of 30 cc citrated blood, (jugular vein), 

 stallion 33. 



x)ecember 3rd. Intramuscular inoculation 30 cc of citrated blood, (jugular 

 vein), stallion 33. 



No secondary swellings occured at the points of inoculati(/n. January 15th. 

 Microscopic examination of peripheral blood and vaginal mucus, negative. Feb. 1st. 

 The animal is in a very poor unthrifty condition, rough staring coat stiff gait. 



March 12. Animal has improved in condition. Vaginal mucus is abundant and 

 milky in appearance. Microscopic examination — The leucocytes and macrophages 

 are degenerated and vacuoled, and there is a very active phagocytosis. 



November 15, 1907. Results of inoculation still doubtful. Trypanosomata have 

 never been detected. In April and July a stiffness of hind limbs was apparent with 

 just a trace of knuckling of fetlocks. There has ever been noted a general air of ill- 

 health and a poorly nourished condition. The genitalia appear normal. 



Animal No. 27. — Two year old filly. ' October 29, 1906, vaginal dischai^e from 

 mare 28 injected into vagina.' — (Dr. S. Hadwen.) 



Inoculations : — 



("Nov. 14 No signs of disease. 



(Nov. 19 Intravenous inoculation 30 cc blood of mare 28. 



- Nov. 21 Intraperitoneal inoculation 25 cc blood citrated of mare 28. 

 Nov. 24 Intraperitoneal inoculation 10 cc blood citrated of mare 28. 

 Dec. 3 Intramusciilrai inoculation 30 cc blood citrated of mare 28. 



Jan. 15. — Microscopic examination, vaginal preparations — negative. 



Jan. 28. — Microscopic examination peripheral blood — negative. 



Condition of animal has not altered, no sign of disease, 4,000 cc of blood with- 

 drawn. 



Jan. 30. — Knees swollen, also fetlock joints. 



