88 DEPARTMENT OF AGRICULTURE 



Jan. 31. — Knees and fetlocks greatly swollen, lameness. Left knee punctured and 

 a quantity of serous fluid slightly tinged with blood collected. Microscopic examina- 

 tion — negative. 



Feb. 6. — Scro-sanguineous fluid drawn from right knee microscopic examination 

 — negative. 



Feb. 20. — Swellings diminished. The gait remains very stiff, more particularly 

 in hind-quarters. Vagina irrigated with citrate solution and mucous membrane 

 scarified, smear preparations — negative. 



Mar. 1. — Swellings have disappeared. Gait still stiff and awkward. 



Mar. 12. — Vaginal blood — negative. 



Nov. 15, 1907. Very similar conditions to the preceding case, (No. 26). On 

 July 27 however in the fluid obtained from a secondary swelling following an ex- 

 perimental injection of a dourine serum, a few developmental trypanosomata were 

 found. While the animals from which the inoculated blood was obtained were un- 

 doubtedly affected with dourine, the parasites of dourine were never found in any 

 of these animals. It is possible that the ' contagium ' present may have lost part of 

 its virulence or again that these native bred fillies may have possessed a certain de- 

 gree of natural resistance to intection. 



Animal No. 39. — A foal about 9 months old, weaned from mare 25 (Seeley 

 mare) showing characteristic symptons of dourine in the last stages: — emaciation 

 and loss of control, hind limbs soiled with, vaginal discharges and urine. 



The foal had always appeared in good health. 



Feb. 11th, 1906, Inoculation No. 1 intramuscular, side of left ribs, 5 c.c. of citrate 

 solution containing a trace of sanguineous fluid from scarified mucous membrane 

 from the vagina of Mare 36. Trj'panosomata present but rare. 



Feb. 17. Inoculation No. 2, intramuscular, flank, 10 c.c. of undiluted blood from 

 peripheral circulation of Mare 36, in which trypanosomata were not demonstrated. 



Feb. 21st. Circular plaque or swelling at seat of inoculation No. 1. Swelling 

 harder at outer border than in centre about five centimetres in diameter, slightly 

 oedematous, painless and a little more heated than the surrounding tissues. 



Trypanosomata were demonstrated in both fresh and stained preparations of 

 the sanguineous fluid drawn from this plaque. Immature and mature organisms 

 recognized, also swollen vacuoled forms undergoing disintegration and staining 

 feebly. On Feb. 22nd., the eleventh day after inoculation, the swelling had slightly 

 increased in size was more oval and oedematous. On the thirteenth day, the third 

 day of the swelling, it became flatter and broader; microscopic preparations were 

 negative on this dat^, also on Feb 25th and March 1st, when the swelling had dis- 

 appeared. March 16th: — no further cutaneous lesions or visible signs of systemic 

 disturbance have been observed to date. 



April 23. — A small cutaneous plaque over the last right rib. Anterior to angle 

 of ileum; the swelling persisting for two days. May 4. — Sub-maxillary lymphatics 

 much enlarged and softened. May 13. — Paralysis of brachial and subscapular nerves, 

 the limb hangs i)endulous from the shoulder or slightly supported on the toe. 



May 20. — Progressing paralysis, knuckling, staggering gait, losing flesh, fever. 



May 27. — Muscular tremors, genitals swollen. 



Frequent attempts at micturition. Urine scanty, mucous membranes anaemic, 

 lymphatics enlarged and softened, emaciation. 



May 29. — 1000 c.c. of blood withdrawn and replaced with normal saline solution. 



June 12. — See photograph Plate XII. 



June 20. — A better appetite and improved muscular control since the openation 

 of May 29. 



June 26. — Condition very grave. The animal is down and helpless. Suppurative 

 processes involving hip and shoulder joints. Genitalia swollen and distorted. The 

 operation of blood letting and Ralino infusion ropeatcd. 



June 27-28. — The animal was able to stand again and appetite returned. 



