DO U RISE OR MALADIE DC CO IT 39 



June 29. — General paralyf^is and death. 



Autopsy. — Body groatly emaciated. Muscles soft, anaemic. Distortion and con- 

 traction of tendons of limbs. Pus in shouder and hip joints. Ascites. Pericarditis. 

 Heart enlarged, muscle soft. vSuprarenal bodies very large, soft, and of an orange 

 colour. Surface of spleen profusely scattered with bright red hsemorraghic spots and 

 patches. Liver large and soft. Estimated weight of animal at time of death, 400 

 lbs. ; heart, 4 lbs. ; spleen, 1 lb. ; liver, 8 lbs. ; kidneys, li lbs. each. 



Trypanosomata were found only on one occasion and in the secondary swelling 

 or plaque, 10 days after inoculation. ISTumerons subsequent examinations of gland 

 juice from enlarged lymphatics, blood from tip of ear, tail and genitalia, and post- 

 mortem smears of the internal organs, failed to reveal any parasites. 



Animal No. 29. — Foal (filly) about 9 months old, weiane<i from mare No. 28 on 

 Janary 1, 1907 (the mare being in the final stages of dourine, viz.: — emaciation, loss 

 of nervous and muscular control, &c.) 



Inoculation No. 1. — February 17, 1907. Intramuscular, flank 10 c.c. citrated 

 blood from general circulation of mare No. 36. 



Inoculation No. 2. — February 22. Subcutaneous, middle right ribs, a titace,of 

 vaginal blood of mare No. 36, diluted in 5 c.c. of citrate solution, active trypanoso- 

 mata being demonstrated in this preparation. 



March 4. — Skin at seat of second inoculation appearg raised and hardened. 

 Microscopic examinations negative. 



March 13. — No further swellings observed, but the animal does not look healthy, 

 being dull, and her movements are sluggish. 



Inoculation No. 3. — March 13, Subcutaneous (2 inches, 5 centimetres anterior 

 to seat of inoculation No. 1), 5 c.c. of vaginal blood from mare No. 36, collected from 

 scarified mucoios membrane after a thorough irrigation of vagina with citrate solu- 

 tion; (trypanosomata being demonstrated in the diluted blood). 



Eight hours later a marked local reaction occurred, the swelling being four in- 

 ches in length, oval shaped and soft, with little heat and no pain. 



March 14. — Swelling flatter and circumscribed; a trace of serous fluid tinged 

 with blood aspirated; microscopic examination negative. 



March 15. — Swelling has persisted. Trypanosomata not detected. 



Inoculation No. J^. — March 23. Scarified mucous membrane of the vagina rub- 

 bing in blood containing trypanosomata from the vagina of mare 36. Trypanosomata 

 were first detected in the vaginal mucus. May 13. The parasites were numerous 

 and were again observed on the following dates, May 27, June 24, July 13, August 

 26, and October 4. The parasites wene never observed in blood taken from a region 

 other than the vaginal mucous membrane. June 17. — Enlargement and softening of 

 submaxillary lympathtics. The gland juice di I not contain trypanosomata. 



November 1.5. — In very good health and condition. A 'slight tumefaction of 

 vulva and a swollen, anaemic vaginal mucous membrane have been the only other signs 

 of the disease visible during thp nine months following infection. Complete ab- 

 sence of depigmentation, vesication, ulceration and nervous phenomena. 



Animal No. 41. — Aged mare not dis<^ased. 



Feb. 17th, 1907. Inoculation, subcutaneous, left ribs 5 c.c. diluted vaginal blood 

 from mare No. 36. 



March 16th. No reaction has been noted either local or general. 



July 7th, 1907. A slight nasal discharge from the right nostril tinged with 

 blood; vaginal secretion abundant and the mucous membrane is swollen. 



Trypanosomata first obtained from the vagina on this date, and subsequently, 

 July 19th. Submaxillary lymphatics much enlarged and softened. 



Aug. 27th. Purulent conjunctivitis of the loft eye. 



