40 DEPARTMENT OF AGRICULTURE 



Sept. 17-20. Bloody nasal discharge and haemorrhagic mucous membranes 

 (following an injection of serum from a dourined stallion — these serum exi)eriments 

 will form the subject of a later report). 



Nov. 15th. Present concfition : — rough, staring coat, poorly nourished body, and 

 general appearance of ill health: but this was also the condition before the experi- 

 mental infection. 



Animal No. 70. — Healthy 2 year old filly. 



Oct. 4th. Inoculation No. 1. Subcutaneous, a trace of blood from vaginal 

 mucous membrane of experimental filly No. 29. 



Oct. 8th. Inoculation No. 2 per vaginal mucous membrane, a trace of blood from 

 vaginal mucTis of experimental filly No. 29. 



Oct. 21. Nov. 15. Trypanosomata in vaginal blood and mucus, often very 

 numerous. The mucous membrane is swollen and anaemic. 



EXPERIMENTAL INOCULATION OF A GELDING. 



Animal No. 43.— A two year old gelding. April 24.-1,000 c.c. of blood with- 

 drawn from jugular vein, followed by a direct transfusion of blood from mare 36, 

 the transfusion lasting 5 minutes. 



July 22. Intermaxillary lymphatics, especially the sublingual are much en- 

 larged and softened. 



Sept. 2. Neuro-muscular inco-ordination, knuckling of hind fetlocks, crepita- 

 tion in hock joints. 



Nov. 15. The foregoing symptoms have appeared in paroxysms and intermissions 

 to date. The body is well nourished and health seems good 



ATTEMPTS AT REINFECTION OF MARES AFFECTED WITH DOURINE. 



Animal No. 9. 'Dot.'' — An experimental mare, the subject of latent dourine. On 

 the floor of the vagina there is a well marked ulcer which has been present for many 

 months without alteration, either in size or appearance. On December 11th, 1906, 

 500 c.c. of blood was drawn from the jugular. 



Feb. 17th, 1907. Intramuscular inoculation, left shoulder, with 10 c.c. of blood 

 from the general circulation of Mare 36, a few drops were also inoculated beneath the 

 vaginal mucous membrane at the same time. 



March 16. No swelling has occured since inoculation. The vaginal mucous 

 membrane appears slightly (Edematous and congested. Microscopic examinations 

 have been made as follows:^ 



November 14. — Peripheral blood — Negative. 



December 11. — Jugular blood; vaginal mucus — Negative. 



January 15. — Vaginal mucus — Negative. 



February 27. — Vaginal blood' — Negative. 



March 7. — Vaginal blood — Negative. 



March 12. — Vaginal blood — Negative. 



May 13. — The whole of the vaginal mucous membrane is very much swollen and 

 cedematous, translucent, of an orange tint, with a few small haemorrhagic spots and 

 papules. Trypanosomata observed in preparations from the mucous membrane on 

 this date, and, subsequently, on May 27 and October 11. 



May 27. — Mucous membrane still more cedematous with prominent infiltrated 

 patches. Depigmented spots appear along muco-cutaneous margin of vulva, their sur- 

 faces rough and * frosted ' or suggestive of a ground-glass surface. In micro-prepar- 

 ations from these latter lesions numerous spirochaeta? were observed. 



