DOURINE OR MALADIE DU COIT 41 



July 16.— Depigmentation spreading to perinaeum. Several ill-defined cicatricea 

 or sears in vaginal mucous membrane. Submaxillary lymphatics enlarged. 



July 27. — Vaginal mucus anaemic, almost white, tough and thickened. 



September 16. — Experimental injection of a dourine serum. A remarkable 

 reaction following: — 



Temperature rose to 103° F., loss of appetite, unwillingness to move, neuro- 

 muscular, inco-ordination. Large oedematous swellings at site of serum injection, the 

 flanks, lower surface of abdomen and mammary glands. 



September 24. — Commencing absorption of swellings, return of appetite and 

 muscular control. 



October 10. — Large oedematous swelling under sterntmi. 



November 15. — Intermittent swellings have continued to date. Paralysis of mus- 

 cles of left eye. An old single ulcer on the floor of the vagina, that has persisted for 

 2 years, still remains. 



Animal No. 7. ' Devil.'. — Experimental mare in latent dourine served by healthy 

 stallion (No. 30), October 10, 1906. No signs of ill health. Eight labium of vulva 

 is greatly atrophied. 



February 22. — Inoculations, intraniusculan, 5 c.c. eitrated vaginal blood from 

 mare No. 36, trypanosomata present in the preparation. On the twelfth day follow- 

 ing, the skin appeared slightly hardened and raised at the seat of inoculation. 

 Mieroscopic examination of the blood smears from this region were all negative. 



March 16. — No cutaneous lesions or systemic disturbances have been noted to 

 date. 



In July and August the submaxillary lymphatics were found to be enlarged and 

 softened. No further change has been noted in this animal. Gland juice and blood 

 has been searched in vain for the dourine parasites. Present condition is healthy. 



Animal No. 17. 'Bells.''— May 27th. Apparently in the best of health. Inocu- 

 lated i)er vaginal mucous membrane with a few drops of blood containing trypanoso- 

 mata of experimental animal No. 29. 



June 27. — Opthalmitis of right eye, a slight milkiness of cornea of left. 



November 15th. — Genitalia and lymphatics normal. Left cornea clear, spreading 

 opacity of right cornea. General condition healthy. 



INOCULATIONS OF DOGS. 



The fact that dogs have proven so satisfactory in attempts to recoveri the causa- 

 tive parasite of this disease, with other observers led to their being used in an 

 attempt toward this end by Dr. Higgins at Ottawa, and Dr. Hadwen at this station 

 during the period he was in charge. 



Following in the footsteps of the various authorities, and of Dns. Higgins and 

 Hadwen, I have used a large number of dogs with a similar end in view. Unfortu- 

 nately, however, no observer on this continent has succeeded in positively transmit- 

 ing the naturally acquired disease to dogs of any age, nor have my experiments been 

 attended with more satisfactory results. 



I append the data secured whch show that, although an infection sufficiently 

 severe to kill the animal or to allow the recovery of the parasite has not occurred, 

 certain symptom.? have been noted in connection with these inoculated animals which 

 are indicated in the table. (Table X.) 



