44 DEPARTMENT OF AGRICULTURE 



Time has not permitted a daily examination of the blood of these dogs; however, 

 numerous microscopic preparations of vaginal blood, blood smears from the tip of 

 the ear, from the tip of the tail and the juice of enlarged glands, have been made 

 at frequent intervals. At the autopsies on these animals, smear and fresh prepara- 

 tions from the spleens, livers and lymphatics have been examined. Trypanosomata 

 have never been observed. 



Dogs 11, 15, 18 and 20 have been subjected to a severe bleeding, in the hope that 

 lessened resistance to the development of infection might result, as has been the case 

 in some of the equines. Blood-letting in the dogs has not resulted in any visible 

 manifestation of the disease. 



Dog No. 18 has been inoculated several times per vaginam, with blood containing 

 trypanosomata. Certain ocular phenomena have been witnessed in dogs Nos. 18 and 

 20. At this date (Nov. 15th) the vulva of No. 18 appears slightly swollen, and the 

 mucous secretion is rather abundant. In No. 20 the penis is swollen and inflamed. 

 There has been a complete absence of oedema in all the dogs. A few have shown 

 enlarged lymphatics, others conjunctivitis. The animals have all been kept on the 

 chain during the i)eriod of captivity, a circumstance that may be responsible for some 

 of these indefinite abnormal conditions. 



KABBIT INOCULATIONS. 



Inoculations, corresponding to tho?e of the dogs have been made with 14 rabbits. 



Two of these animals died within 24 hours of septicaemia and two were accident- 

 ally killed. The remainder are alive and apparently in the best of health. No signs 

 of disease have ever been noted. Numerous blood examinations negative. 



The only suspicious indications of a dourine- infection lie in a few abnormal 

 conditions of the eyes. Conjunctivitis, with, or without a slight discharge. A slight 

 milkiness of the cornea, fugitive in character, with one exception, a rabbit which 

 has become blind in one eye, with ex-ophthalmia. Three rabbits have been destroyed, 

 the remainder survive, and api)ear healthy. Oedema never noted. 



MICE INOCULATIONS. 



Thirty field mice have been inoculated, five of which vlied within a few hours of 

 septicemia. 



March 31, 1907. Two mice inoculated with sediment of (edematous fluid of stal- 

 ion 33 are still living, two and a half months after inoculation. 



Of three mice inoculated with the sediment of serous fluid from the knee swellings 

 of filly No. 27, one died on the 4th day and the other two are still living, six weeks 

 after inoculation. Curiously, at intervals of 10-15 days, they were found in an ex- 

 tremely lethargic condition, the eyes were closed and breathing was rapid. After 

 several hours in this condition they recovered and soon became as active as ever. 



Of two mice inoculated with sero-sanguineous fluid containing the Trypanosoma 

 equiperdnim from the inoculation plaque of animal No. 39, one died on the twelfth day 

 in the lethargic condition described above, while its mate, after being in the same con- 

 dition for eight hours, recovered and is still alive. Of those inoculated from mare 36 

 {Trypansoma equiperdum demonstrated in the preparation), three died within three 

 days, one on the tenth day and the others are still living, but the most of those surviv- 

 ing have had one or more attacks of the coma or paralysis described above. Of six 

 control mice, one has died and none have ever been found in the comatose condition. 

 One mou^e inoculated with the ccntrifugalized sediment of the ascitic fluid of mare 

 28, died on the seventh flay, and in a blood smear from this mouse an hour before 

 death, six trypansomata were observed, which possessed some of the characteristics 

 of the T. equip'erdum 



