38 MEDICAL BACTERIOLOGY 



STERILIZATION TABLES 



HOT AIR 



Material Temperature Time, hours 



Flasks, bottles, test-tubes, etc., stoppered with cotton or wrapped 

 in paper 1 5oC. i to 3 



Flasks, petri dishes, tubes, ampules, pipettes and other utensils, 

 not injured by heat, not stoppered nor wrapped with inflam- 

 mable material '. 200 to 3ooC. i to 2 



Talc, isoC i to 3 hours 



Kaolin, i5oC i to 3 hours 



Boric acid, i5oC. i to 3 hours 



Zinc oxide, i5oC i to 3 hours 







(Powders should be spread in a stratum not more than % inch thick.) 



AUTOCLAVE 15 POUNDS PRESSURE FOR 20 MINUTES 



Water Plain bouillon 



Salt solution Plain agar 



Oils Glycerin bouillon 



Milk Glycerin agar 



Potato Mercury sozoiodolate 



Mercury Salicylate 



STEAM (ioo J C.) 



Material Time, minutes Np. of days 



Gelatin 15 3 



Bouillon and agar containing sugar 15 3 



Sugars 15 3 



Litmus milk 15 3 



Adrenalin 15 3 



Argyrol 15 3 



Arsenic 15 3 



Atropin 15 3 



Caffeine 15 3 



Calomel cream 15 3 



Gray oil 15 3 



Gums 15 3 



Mercury benzoate 15 3 



Mercury cacodylate 15 3 



Morphine 15 3 



Paraffin 15 3 



Sodium cacodylate 15 3 



Stovaine 15 3 



Straphantin 15 3 



Endo's medium 15 3 



MacConkey's bile-salt medium 10 a 



Alkaloids, generally 10 2 



