CHAPTER VI 

 CULTURE MEDIA 



Culture media are sterile fluids and solids upon which bacteria are cultivated 

 to determine the character of their growth, to study and classify species, to iso- 

 late some organisms from others, to detect the presence, number and kind of 

 bacteria in water, milk, etc., and to obtain large numbers of certain organisms 

 for the preparation of diagnostic and therapeutic agents, such as bacterial 

 vaccines. 



For convenience of description, culture media may be divided into two 

 classes, natural and artificial. 



NATURAL CULTURE MEDIA 

 Fluid Solid 



Milk 



Whey 



Blood serum 



Serous fluids 



Eggs 



Ox bile 



Urine 



Blood serum 



Eggs 



Potato 



ARTIFICIAL CULTURE MEDIA 



Fluid 



Bouillon 

 Lactose bouillon 

 Glucose bouillon 

 Saccharose bouillon 



With or without litmus 

 Other sugar bouillons 

 Glycerin bouillon 

 Ox-bile bouillon 

 Dunham's solution 

 MacConkey's bile-salt-medium 

 Trypsinized-peptone-water 



Solid 



Agar 



Lactose agar 

 Glucose agar 

 Saccharose agar 



With or without litmus 

 Other sugar agar media 

 Glycerin agar 

 Blood-smeared agar 

 Endo's agar 

 Russell's agar 

 Malachite-green agar 

 Gelatin media 

 PetrofFs medium 



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