WASSERMANN AND OTHER COMPLEMENT FIXATION TESTS 22Q 



contents and the tube is then inverted and shaken a sufficient number of time 

 to evenly distribute the cells throughout the fluid; the tubes are again centrifu- 

 gahzed until all the cells settle to the bottom; the salt solution is pipetted off 

 This washing is repeated twice. After the third washing the cells are ready to 

 be injected into the rabbit. 



Only large healthy rabbits should be used. With a pair of scissors the hair 

 is removed so as to expose one of the veins on the margin of the ear. Half a 



A 



FIG. 37. 



A, Sterilized glass bottle with wide mouth and ground glass stopper, half filled with 

 sodium citrate solution. B, Blood from sheep flowing into bottle (C) until the bottle is 



three-fourths full. 



cubic centimeter of red blood cells is poured into a small (2 cc. capacity) Luer 



syringe, and with the finest needle through which the cells will pass, they are 



injected into the vein (Fig. 39). 



Twenty-four hours after the first injection, in exactly the same manner, the 



second is given, the quantity of red blood cells injected being i cc. 



Twenty-four hours after the second injection a third is given quant it \ 

 Twenty-four hours after the third injection the last one is given quantity 



3 cc. 



