E. E. Nelson and C. W. Greene 



55 



Creatine was found in gar eggs in amounts varying from traces 

 up to slightly less than 10 mg. per 100 gm. of moist sample. No 

 especial significance is attached to this observation. 



The Inorganic Ash and Water. 



Our data show variations of the ash from 0.25 to 0.59 per cent, 

 a rather extreme variation, for which there is no obvious explanation. 



Water determinations were made directly on separate samples 

 of the first seven fish but only indirectly on the others. The 



TABLE III. 



The Protein, Water, and Extractive Fractions Figured on a Fat-Free Basis, 

 and Arranged in Series According to the Weights of the Ovaries. 



* The organic extractives were obtained by subtracting the ash from 

 the water-soluble solids. 



f Not recorded, but the weight was several hundred grams. 



water determination by differences throws the cumulative error 

 on this fraction. Nevertheless, the agreement is quite close as 

 between the indirect and the direct determinations on the first 

 seven specimens. 



General Comparison. 



We hoped to be able to compare the composition of the gar ovaries 

 at different stages of development. However, these fish apparently 

 spawn over a long season and there is no way of determining the 

 degree of development except by the general appearance and the 

 variation in size of the ovules. In our youngest specimen, Cll, 



