50 Ovaries of the Gar, Lepidosteus 



was placed in a similar flask and treated with NaOH and picric- 

 acid in the same manner. At the end of 10 minutes both flasks 

 were filled to the mark with distilled water and the readings 

 taken in a Duboscq colorimeter. All determinations were made 

 by daylight. The standard creatinine was isolated from urine 

 by Benedict's method (1914) and checked against a sample of 

 pure creatinine from Dr. Myers' laboratory. The values as 

 recorded in the tables of this paper are in terms of creatine ob- 

 tained by multiplying the creatinine determination by the factor 

 1.16. 



The amino nitrogen of the extractives was obtained by the 

 method of Van Slyke (1912) using the micro-apparatus. 1 



Protocols with Descriptive Data for Chemical Samples. 



Sample Cll. Fish 18. Lepidosteus platystomus ovary. Length of fish 

 52 cm., weight 385 gm. Weight of ovaries 15.9 gm., diameter of ova 1.2 mm. 

 Weight of chemical sample 11.908 gm. Fairport, Iowa, July 25, 1917. 



Sample C20. Fish 19. Lepidosteus platystomus ovary. Length of fish 

 52 cm., weight 435 gm. Weight of ovaries 30.5 gm., diameter of ova varied 

 to a marked degree. "Many small white immature ova." Largest ova 

 2.2 mm., smallest 0.8 mm. Weight of chemical sample 18.464 gm. Fair- 

 port, Iowa, July 25, 1917. 



Sample C22. Fish 32. Lepidosteus platystomus ovary. Length of fish 



56.3 cm., weight 535 gm. Weight of ovaries 48.3 gm., diameter of ova 1.7 

 mm. Weight of chemical sample 17.858 gm. New Boston, Illinois, August 

 25, 1917. 



Sample C26. Fish 29. Lepidosteus platystomus ovary. Ovary weight 

 27 gm., diameter of ova 1.5 mm. "Ovary filled with red fat." Weight of 

 chemical sample 20.958 gm. Fairport, Iowa, August 16, 1917. 



Sample C29. Fish 35. Lepidosteus platystomus ovary. Length of fish 

 57.5 cm., weight 504 gm. Weight of ovaries 48.5 gm., diameter of ova 1.9 

 mm. Weight of chemical sample 20.808 gm. New Boston, Illinois, August 

 25, 1917. 



Sample C36. Fish 33. Lepidosteus platystomus ovary. Length of fish 

 58 cm., weight 611 gm. Weight of ovaries 52.8 gm., diameter of ova 1.8 

 mm. Weight of chemical sample 20.373 gm. New Boston, Illinois, August 

 25, 1917. 



Sample CSS. Fish 34. Lepidosteus platystomus ovary. Length of fish 



55.4 cm., weight 619 gm. Weight of ovaries 72.5 gm., diameter of ova 

 2.0 mm. Weight of chemical sample 20.059 gm. New Boston, Illinois, 

 August 25, 1917. 



1 All total nitrogen and amino nitrogen determinations were made f or- 

 us by Mr. Louis Gambee. 



