156 MODERN BIOLOGIC THERAPEUSIS 



Bruck, Neisser and Sachs and others, to demon- 

 strate the presence of immune bodies in various 

 sera. When used for the diagnosis of syphilis, 

 this test is termed the "Wassermann Reac- 

 tion." 



Essential Factors As pointed out by Kol- 

 mer, the following factors are essential to the 

 successful carrying out of the complement fixa- 

 tion test: (1) Reliable reagents, particularly a 

 good antigen, must be used; for good results 

 cannot be secured with indifferent reagents, no 

 matter how much care is exercised; (2) There 

 must be an accurate adjustment of the hemolytic 

 system; (3) The observer must possess a thor- 

 ough working understanding of the underlying 

 principles and the quantitative relations of the 

 various reagents; and (4) He must have a care- 

 ful, painstaking and accurate habit of pipeting 

 small amounts. 



TECHNIC OF COMPLEMENT FIXATION TEST 



Five substances are required to carry out the 

 test: (1) Immune serum, (2) Antigen, (3) Com- 

 plement, (4) Hemolytic amboceptor, and (5) 

 Red Cell suspension. 



Immune Serum The method of withdrawing 



