METHODS OF EMBEDDING. 



297 



The thin coating of glycerin on the inside of the embedding-dish 

 serves the purpose of preventing the collodion from sticking to the 

 glass. 



2. Embedding in Paraffin. — The specimen should first be trimmed 

 so as to have one surface parallel to the plane of the future sections. 

 This trimming had best be done before the specimen is placed in the 

 first paraffin bath. When specimens are to be embedded directly 

 from the paraffin bath, the simplest method is to employ a small box 

 made from rather heavy writing-paper by folding it in the follow- 

 ing way (Fig. 260) : Suppose the box is to be 2 inches long, 1 inch 



Fig. 260. 



(3) 



(3) 



C D 



The upper half of the diagram represents the creases in the paper which are produced by 

 folding; the lower half shows the last fold on the right and the next to the last on the 

 left. The bottom of the box is the rectangle A, B, C, D. 



wide and £ inch deep. Cut a piece of paper 2^- inches wide 

 (= width of box + twice its depth) and 4^- inches long (= length 

 of box + twice its depth + 1 inch to allow ^ inch fold at each end). 

 Crease the paper, longitudinally, f inch from and parallel to the edge 

 by folding and pressure (folds 1-C and 1-D). Then open these 



