BORIC ACID UPON NUTRITION. 



The carbohydrate element in the diet, as already stated, was 

 supplied by commercial cracker dust. This was purchased in 

 large quantity and preserved hi well stoppered bottles. It 

 contained on an average 1.46 per cent of nitrogen. The lard 

 employed was entirely free from any recognizable amount of 

 nitrogen. 



The daily diet was divided into two equal portions, one-half 

 being fed at 8 A.M. and the other half at 6 P.M. When borax 

 or boric acid was given, the daily dose was likewise divided 

 and given either with the food or directly after. The body- 

 weight of the animal was taken each morning just before 

 feeding. Each day's urine included the fluid passed from 

 8 A.M. to 8 A.M. of the next day. 



Methods of Analysis. Nitrogen was determined wholly by 

 the Kjeldahl method, viz., in the daily analyses of the urine, 

 faeces, and food material. All analyses were made in dupli- 

 cate, and the figures given are based upon the average of 

 closely agreeing results. In analysis of the urine 5 c.c. were 

 used for each determination, oxidation being carried out in a 

 long-necked Kjeldahl flask with 10 c.c. of sulphuric acid and 

 a crystal of cupric sulphate, thus doing away with the necessity 

 of adding sodium sulphide hi the distillation. The ammonia 



