BORIC ACID UPON NUTRITION. 9 



Combined sulphuric acid was determined by Baumann's 

 method, using 100 c.c. of urine.* 



Chlorine was determined in 10 c.c. of urine by Neubauer 

 and Salkowski's modification of Mohr's method. f Other 

 methods occasionally made use of are referred to in their 

 appropriate place. 



First Experiment. With Borax. The animal made US6 of 

 in this experiment was a short-haired mongrel bitch weighing 

 about 12 kilos. She was brought into a condition approxi- 

 mating to nitrogenous equilibrium only after a preliminary 

 period of nearly three weeks, during which time superfluous 

 fat was lost and she became wholly accustomed to her sur- 

 roundings. The daily food at the tune the experiment actu- 

 ally commenced, consisted of 250 grams of the prepared meat, 

 70 grams of cracker dust, 40 grams of lard, and 500 c.c. of 

 water. It contained 9.814 grams of nitrogen. This diet, 

 with the above content of nitrogen, was adhered to through- 

 out the entire experiment of twenty-seven days, the only 

 variation being the slight changes in the amount of nitrogen, 

 to be seen in the tables, incidental to the use of different lots 

 of meat and in the employment of gelatin capsules during the 

 borax period. These gelatin capsules, in which the borax 

 was administered, contained 14.95 per cent of nitrogen, the 

 four capsules used each day during the borax period contain- 

 ing 0.12 gram of nitrogen. This amount was naturally in- 

 cluded in the nitrogen of the food. 



The experiment extended through twenty-seven days and 

 was divided into three periods of nine days each: a fore or 

 normal period during which no borax was given, a borax 

 period during which 45 grams of borax (5 grams a day) were 

 administered, and an after period when normal conditions 

 again prevailed. During the borax period of nine days the 

 quantity of borax given per day amounted to nearly 0.6 per 

 cent of the total food and drink ingested, while of the solid 

 food it formed 1.3 per cent. This dosage of borax, consider- 

 ing the size of the animal, was fairly large, and with this 

 * Ibid., p. 447. t Ibid. p. 437. 



