24 INFLUENCE OF BORAX AND 



acid through the urine as was observed by Forster; our 

 results, indeed, fail to show any distinct influence exerted by 

 boric acid upon the metabolism of phosphorized matter. 



Third Experiment. With Borax and Boric Acid This 



experiment was divided into seven periods of eight days each, 

 thus making a total of fifty-six consecutive days during which 

 the variations in the composition of the urine and faeces were 

 followed as before, under the influence of both borax and 

 boric acid. The object in extending the experiment through 

 this lengthy period was to ascertain whether prolonged 

 treatment with borax and boric acid might not eventually 

 result in such a disturbance of physiological equilibrium that 

 more positive data would be obtained. "With this end in 

 view, a mongrel bitch of ten kilos body-weight was brought 

 into nitrogenous equilibrium, after which the urine and faeces 

 were analyzed for eight consecutive days, i. e., the fore period. 

 Borax was then given with the food for eight days, making 

 the first borax period. This was followed by another period 

 of eight days during which neither borax nor boric acid were 

 administered, after which came a third period of eight days 

 when boric acid was fed. This, in turn, was succeeded by a 

 normal period of equal length, followed by eight days of borax 

 treatment the second borax period concluding with a final 

 after period of eight days, i. e., a total of fifty-six days. By 

 thus keeping the same animal under continuous observation 

 for this length of time it might reasonably be expected that 

 any cumulative action assuming it to exist would be 

 clearly manifest. Further, considerably larger daily doses of 

 borax and boric acid were administered than in the preceding 

 experiments. 



The daily diet made use of throughout the entire experiment 

 consisted of 160 grams of the prepared meat, 40 grams of 

 cracker dust, 30 grams of lard, and 430 c.c. of water. Its 

 exact content of nitrogen is shown in the table of the fore 

 period. The total amount of nitrogen ingested during the 

 fore period was 52.163 grams. The amount excreted during 

 the same period was 51.734 grams, thus showing a nitrogen 



