46 THE MUCIN OF WHITE 



and fluffy. The yield amounted to twelve grams of the dry 

 product, and, assuming that the entire amount of mucin had 

 been extracted from the tendons, and disregarding the loss 

 incidental to purification, this quantity would imply the 

 presence in the fresh tendons of 1 per cent of mucin. 



The composition of the product, dried at 110 C. until of 

 constant weight, was as follows : * 



PREPARATION No. 1. 



I. 0.2670 gram of substance gave 0.4781 gram of CO 2 = 48.84 per cent 



C, and 0.1585 gram of H^O = 6.60 per cent H. 

 IL 05277 gram of substance gave 0.4082 gram of CO 2 = 48.89 per cent 



C, and 0.1329 gram of H a O = 6.48 per cent H. 



HI. 0.1975 gram of substance gave 0.3548 gram of C0 2 = 48.99 per cent C. 

 IV. 05363 gram of substance gave 0.1417 gram of H-jO = 6.66 per cent H. 

 V. 0.2426 gram of substance gave, by the Kjeldahl method, 0.02865 gram 



of nitrogen = 11.81 per cent N. 

 VL 0.2764 gram of substance gave, by the Kjeldahl method, 0.03246 gram 



of nitrogen = 11.79 per cent N. 

 VII. 0.2784 gram of substance gave, by the absolute method, 27.63 c. c. of 



nitrogen at 13.3 C., and 764.7 mm. pressure = 11.96 per cent N. 

 Vm. 0.3345 gram of substance gave, by the absolute method, 33.3 c. c. of 



nitrogen at 13.2 C., and 754.6 mm. pressure = 11.84 per cent N. 

 IX. 0.5373 gram of substance gave, by fusion with NaOH + KNO W 0.0943 

 gram of BaSO 4 = 2.41 per cent S ; after deducting sulphur of ash 

 = 2.36 per cent S. 



X. 0.4969 gram of substance gave, by fusion with NaOH + KNO 8 , 0.0856 

 gram of BaSO 4 = 2.37 per cent S ; after deducting sulphur of ash 

 = 2.32 per cent S. 



XI. 0.2943 gram of substance gave 0.0023 gram of ash = 0.78 per cent ash. 

 XIL Ash from 0.2943 gram of substance gave 0.00112 gram of BaSO 4 = 0.05 

 per cent S. 



PERCENTAGE COMPOSITION OF THE ASH-FREE SUBSTANCE. 



Average. 



C 49.22 49.27 49.87 49.29 



H 6.66 6.64 ... 6.71 6.63 



N 11.90 11.88 12.05 11.93 11.94 



S 2.36 2.32 2.34 



O 29.80 



100.00 



* The nitrogen was determined by both the absolute and the Kjeldahl 

 method, while carbon and hydrogen were determined by combustion in 

 oxygen gas in an open tube, the products of combustion passing over a layer 

 of cupric oxide, chromate of lead, and metallic copper. 



