48 THE MUCIN OF WHITE 



half-saturated lime-water are required for every gram of 

 tissue in order to insure a complete extraction. Thus, after 

 the second extraction of the above 1700 grams of tissue, 

 a third extraction was made, using again three liters of 

 half-saturated lime-water. This solution, on treatment with 

 hydrochloric acid, gave a precipitate weighing one to two 

 grams when purified, but it was noticeable that more acid 

 was required in order to effect a good flocculent separation of 

 the mucin. Even with a fourth extraction of the tissue a 

 little mucin was obtained, showing as a decided turbidity 

 when the alkaline fluid was made distinctly acid, but it was 

 not until four or five days' standing that a distinct precipitate 

 settled out even on the addition of stronger hydrochloric acid. 

 The amount so obtained, however, was very small. 



The composition of the main product obtained from the 

 1700 grams of tissue when dried at 110 C., until of con- 

 stant weight, was as follows : 



PREPARATION No. 2. 



L 0.3194 gram of substance gave 0.5659 gram of CO 2 = 48.32 per cent C, 



and 0.1815 gram of H 2 = 6.31 per cent H. 

 IL 0.4197 gram of substance gave 0.7471 gram of CO S = 48.54 per cent C, 



and 0.2446 gram of H 2 O = 6.47 per cent H. 

 IIL 0.4051 gram of substance gave 0.7189 gram of C0 a = 48.39 per cent C, 



and 0.2353 gram of H 2 O = 6.45 per cent H. 

 IV. 0.2519 gram of substance gave, by the Kjeldahl method, 0.02965 gram 



of nitrogen = 11. 7 7 per cent N. 

 V. 0.2578 gram of substance gave, by the Kjeldahl method, 0.03026 gram 



of nitrogen = 11.74 per cent N. 

 VL 0.2954 gram of substance gave, by the Kjeldahl method, 0.03446 gram 



of nitrogen = 11.67 per cent N. 

 "VTL 0.6610 gram of substance gave, by fusion with NaOH+KN0 8 , 0.1131 



gram of BaS0 4 =2.35 per cent S; after deducting sulphur of ash = 



2.32 per cent S. 

 VIII. 0.6248 gram of substance gave, by fusion with NaOH+KN0 8 , 0.0936 



gram of BaS0 4 = 2.45 per cent S; after deducting sulphur of aah = 



2.42 per cent S. 

 IX. 0.6724 gram of substance gave, by fusion with NaOH+KNO 8 , 0.1140 



gram of BaSO 4 = 2.33 per cent S ; after deducting sulphur of ash = 



2.30 per cent S. 



X. 0.3735 gram of substance gave 0.0025 gram of ash =0.67 per cent ash. 

 XL Ash from 0.3735 gram of substance gave 0.00082 gram of BaS0 4 =0.03 



per cent 8. 



