FIBROUS CONNECTIVE TISSUE. 49 



PERCENTAGE COMPOSITION OF THE ASH-FKEE SUBSTANCE. 



Average. 



C 48.64 48.87 48.72 48.74 



H 6.36 6.52 6.60 6.46 



N 11.85 11.82 11.74 11.80 



S 2.32 2.42 2.30 2.35 



O 30.65 



100.00 



A third specimen of mucin was prepared as follows : Fifteen 

 hundred grams of ox tendons were finely divided, the tissue 

 extracted for twenty-four hours with three liters of 10 per 

 cent salt solution, and then with water until the salt was 

 wholly removed. The tissue was next extracted for sixty 

 hours with three liters of half-saturated lime-water. From this 

 solution the mucin could be only partially separated by the 

 addition of 0.2 per cent hydrochloric acid, quite a quantity of 

 10 per cent acid being required to effect a flocculent precipi- 

 tation of the substance. This was purified by itself and not 

 subjected to analysis. The tendons were again extracted with 

 three liters of half-saturated lime-water for forty-eight hours, 

 and from this solution the mucin was separated as a flocculent 

 precipitate by the addition of 0.2 per cent hydrochloric acid. 

 This precipitate was purified by washing with 0.2 per cent 

 hydrochloric acid, solution in 0.5 per cent sodium carbonate, 

 reprecipitation with 0.2 per cent hydrochloric acid, etc., as 

 described under the last preparation. The yield of dry product 

 from this second extraction of the tissue with lime-water 

 amounted to 6.5 grams. Dried at 110 C. until of constant 

 weight, this preparation gave the following results on analysis : 



PREPARATION No. 3. 



I. 0.3598 gram of substance gave 0.6292 gram of C0 2 = 47.69 per cent C, 



and 0.2072 gram of H 2 = 6.40 per cent H. 



II. 0.2939 gram of substance gave 0.5150 gram of CO 2 = 47.79 per cent C, 

 and 0.1725 gram H 2 = 6.52 per cent H. 



III. 0.3154 gram of substance gave 0.6536 gram of C0 2 = 47.87 per 



cent C. 



IV. 0.1644 gram of substance gave 0.0944 gram of H 2 O = 6.38 per cent H. 

 V. 0.1965 gram of substance gave, b7 the Kjeldahl method, 0.02255 gram 



of nitrogen = 11.47 per cent N. 



4 



