OF ALCOHOL UPON DIGESTION. 



75 



10 c. c. of the fluid studied, and allowing it to remain there 

 for an instant previous to swallowing it. The normal con- 

 ditions were thus closely imitated, and reflex influences from 

 the stomach not excluded. The head was now turned to one 

 side and rested upon the arm, the saliva being allowed to 

 collect in the cavity of the mouth. As the fluid accumulated 

 it was from time to tune, during fifteen to twenty minutes, 

 allowed to flow out of a corner of the mouth into a measuring 

 vessel. Movements of the jaws and tongue were carefully 

 avoided and psychic stimulation was excluded as far as 

 possible. The method, already recommended by Hofbauer,* 

 was found to be reasonably satisfactory, and control trials 

 showed that the quantities of saliva obtained within periods of 

 fifteen or twenty minutes could be appropriately compared. 



Of the saliva thus collected, 3-4 c.c. were taken for analysis. 

 A weighed quantity was dried in a tared crucible on a water- 

 bath and then for four or five hours at 105 C., this tune being 

 found sufficient to bring crucible and contents to a constant 

 weight. Total solids were thus determined. The crucible 

 was then ignited, care being taken to prevent loss by volatili- 

 zation of salts. The ash thus obtained is given as salts in the 

 protocols, while the organic constituents were obtained by 

 subtracting the amount of salts from the total solids. In 

 some cases the amount of chlorine in the ash was determined by 

 the usual method of titration with weak silver nitrate solution. 

 The analytical results are all expressed in percentages. The 

 following figures serve to illustrate the results of a typical 

 duplicate analysis : 



SUBMAXILLARY SALIVA OF DOG. 



Hofbauer, Archly f iir die gee. Physiol., 1897, IXT, p. 503. 



