132 



THE ROTARY PROPERTIES OF 



Ammonium-sulphate Solutions. 



The hemp-seed globulin was more soluble in a 10 per cent 

 ammonium-sulphate solution than in the preceding sodium- 

 sulphate solution, but less soluble than in a 10 per cent 

 sodium-chloride solution. 



Two independent solutions of the globulin in a 10 per cent 

 ammonium-sulphate solution were made, and the following 

 results obtained from them: 



These results, although not very concordant or reliable, are 

 sufficiently so to show that the specific rotation of hemp-seed 

 globulin in an ammonium-sulphate solution lies between the 

 values found respectively for sodium-sulphate and sodium- 

 chloride solutions. 



The weighted mean of the above gives for the specific 

 rotation of hemp-seed globulin in a 10 per cent ammonium- 

 sulphate solution, 



(a) D = -39.8. 



Sodium-chloride-potassium -hydroxide Solution. 



The remnants of Solutions 3 and 4 (hemp-seed globulin in a 

 10 per cent sodium-chloride solution) were used to form an 

 alkaline solution by adding to them a definite volume of an 

 8.2 per cent potassium-hydroxide solution ; 100 cubic centi- 

 meters of the solution thus formed contained 0.766 gram 

 of hemp-seed globulin, 9 grams of sodium chloride, and 0.82 

 gram of potassium hydroxide. 



Five measurements of the rotation due to this solution gave 

 a mean angle of 0.371 0.011 for a 100-millimeter tube, 

 the average temperature of the solution being 22.1 C. The 



