SOME VEGETABLE PROTEIDS. 



133 



specific rotation of the hemp-seed globulin calculated from 



this is 



(a) D = -57.6 1.4. 



After these measurements the solution was heated to 40 C. 

 for fifteen or twenty minutes to insure the complete conver- 

 sion of the proteid into an alkali-albumin, and, after cooling, 

 its rotary power was again determined. 



Four sets of observations with a 100-millimeter tube gave a 

 mean rotation of 0.412 0.011, the average temperature 

 of the solution being 21.2 C. The specific rotation of the 

 globulin calculated from this 



(a) D = -61.6 1.4. 



This is only 4 greater than the value obtained before 

 warming the solution, which would seemingly indicate that 

 the proteid must have been almost entirely converted into an 

 alkali-albumin before the first determination of the specific 

 rotation was made. 



Dilute Potassium-hydroxide Solution. 



Two determinations of the amount of moisture in the air- 

 dry hemp-seed globulin were made at different times by drying 

 it at 110 C. The first determination gave a loss of weight of 

 0.0654 gram for each gram of globulin, and the second 

 of 0.0659 gram, showing that the percentage of moisture 

 remained practically unchanged. 



3.3720 grams of this air-dry globulin were dissolved hi 

 an 0.18 per cent KOH solution, and made up with the same 

 solution to 200 cubic centimeters. The rotary power of this 

 solution of hemp-seed globulin was determined immediately 

 after making it, and again after heating it at 40 or 50 C. for 

 some thirty to forty minutes, with the following results : 



