134 



THE ROTARY PROPERTIES OF 



The values found for (a) D agree within the limit of error of 

 observation, and are slightly larger than those obtained for 

 hemp-seed globulin in the last solution. Their agreement 

 would indicate that the action of the alkali in raising the 

 rotary power of the globulin is exerted at once and without 

 the aid of heat, the proteid being presumably changed more or 

 less completely into an alkali-albumin when dissolved in the 

 alkaline solution. 



Dilute Sodium-carbonate Solution. 



5.6987 grams of the air-dry hemp-seed globulin were dis- 

 solved in a 0.2 per cent sodium-carbonate solution and then 

 made up with the same sodium-carbonate solution to 240 cubic 

 centimeters. The rotary power of this globulin solution was 

 determined, (1) immediately after making up the solution, (2) 

 six hours after, and (3) after heating it at 40 to 50 C. thirty 

 or forty minutes, and cooling. The results of the polarimeter 

 observations were as follows: 



These show a slight increase in the specific rotation during 

 the first six hours, and a marked increase after heating the 

 solution of over 4. The globulin is evidently not so readily 

 nor so completely transformed by the sodium carbonate as by 

 the stronger caustic alkali. 



Dilute Hydrochloric-acid Solution. 



3.6906 grams of the air-dry hemp-seed globulin were dis- 

 solved in a 0.26 per cent hydrochloric-acid solution and made 

 up to 262 cubic centimeters with the same solution. This 

 solution was exceptionally transparent and the most satisfac- 



