SOME VEGETABLE PROTEIDS. 



135 



tory for use in the polarimeter of any proteid solution tried by 

 the writer. Its rotary power was determined, (1) two hours 

 after making the solution up, (2) twenty hours after making 

 it up, (3) after heating it at about 40 C. for twenty minutes, 

 and (4) after letting it stand for thirty-four days. The results 

 of the polarimeter observations were as follows : 



The largest value obtained for the specific rotation was 

 within two hours after making the solution up and before 

 heating it. That obtained twenty hours after was some 4 

 lower, and there was only a slight increase in the specific ro- 

 tation after the solution had been heated. After the solution 

 had stood for over a month the specific rotation was found to 

 have increased about 1.7. 



III. FLAX-SEED GLOBULIN. 

 Preparation and Composition. 



On account of the gummy character of the extracts from 

 flax-seed, and the consequent difficulty hi filtering them, it was 

 not easy to obtain this proteid in large quantities. That used 

 in this investigation was prepared by the following method : 



Ordinary flax-seed meal was treated with benzine (petroleum 

 ether) to extract the fat, and then, after drying, was passed 

 through a fine sieve to remove the husk as far as possible. 

 The globulin was extracted from this prepared meal by means 

 of a 10 per cent sodium-chloride solution and the extract fil- 

 tered clear. When extracted at 60 C. the extract obtained 

 was richer in globulin but more difficult to filter than when 

 extracted at 20 C. Extracts were obtained at both tempera- 



