140 



THE ROTARY PROPERTIES OF 



This globulin from the Brazil nut has been analyzed by 

 Sacchse, Ritthausen, and others.* The following analyses are 

 given by Osborne : 



The writer found the percentage of ash to be 0.42 per cent 

 in the globulin preparation obtained by cooling a hot aqueous 

 extract of the Brazil-nut meal. 



Sodium-chloride Solutions. 



The globulin from the Brazil nut was quite soluble in a 10 

 per cent sodium-chloride solution, forming a rather dense 

 opaque fluid so opaque to sodium light that it was dif- 

 ficult to measure its specific rotation, even with solutions 

 containing from 0.5 to 1.0 per cent of the proteid. The 

 opacity of its solutions and the limited quantities in which 

 this globulin could be prepared prevented an extensive in- 

 vestigation of its rotary properties. As with the flax-seed 

 globulin, the specific rotation of the Brazil-nut globulin was 

 only determined for solutions with sodium chloride. 



Three solutions of this globulin in a 10 per cent sodium- 

 chloride solution were prepared. The crystallized globulin 

 used in solutions numbered 17 and 19 was obtained by cooling 

 a hot (60 C.) water extract of fat-free Brazil-nut meal. 

 That used in Solution 18 was obtained by dialysis from a cold 

 (20 C.) 10 per cent sodium-chloride extract of the Brazil- 

 nut meal. This last solution was of a whitish color and 

 much more opaque to sodium light than the others. After 

 repeated dilution a measurement of its rotary power was 



American Chemical Journal, vol. xiv, p. 669. 



