PAPAIN-PROTEOLYSIS. 175 



40 C. in the presence of chloroform. The resultant fluid 

 freed from insoluble matter and neutralization precipitate was 

 concentrated to a small volume and the albumoses precipitated 

 by saturation with ammonium sulphate, boiling hot, from a 

 neutral, acid, and alkaline reacting fluid. The precipitate so 

 obtained was dissolved in water, the fluid carefully neutralized, 

 and then dialyzed in running water until wholly free from 

 ammonium sulphate and other salts. The solution was then 

 filtered from a little insoluble matter (heteroalbumose, dysal- 

 bumose) concentrated to a small volume, and a portion tested 

 for protoalbumose by saturation of the neutral fluid with rock 

 salt. No precipitate whatever was obtained, consequently the 

 entire volume of fluid was brought to a syrup and the deutero- 

 albumose precipitated with strong alcohol. After thorough 

 washing with alcohol and ether, the substance was dried at 

 100 C. making about 20 grams of pure deuteroalbumose. 



To obtain the peptone, the ammonium sulphate-saturated 

 filtrate from the albumoses was treated with 50 per cent 

 alcohol, thereby precipitating a large portion of the ammonium 

 salt, while the residual sulphate was removed from the filtrate, 

 after freeing from alcohol, by treatment with barium hydroxide 

 followed by barium carbonate. On- evaporating the final 

 filtrate to a syrup and treating with alcohol, the peptone was 

 precipitated more or less gummy, after which it was dehydrated 

 by successive treatments with absolute alcohol and ether, and 

 finally dried at 100 C. About 10 grams of pure peptone were 

 obtained. 



Mode of Experimentation. Our study of the physiological 

 action of the deuteroalbumose and peptone formed above was 

 limited to ascertaining their effects on blood-coagulation, their 

 influence on blood-pressure, and their elimination by the 

 kidneys. In all of the experiments dogs were employed, the 

 animals always being anaesthetized. Most generally this was 

 accomplished by means of a mixture of equal parts of chloro- 

 form and ether, although in some of the experiments morphine 

 sulphate was injected hypodermically followed by the admin- 

 istration of chloroform and ether. In the few cases where 



