176 PAPAIN-PROTEOLYSIS. 



morphine was used, it was employed in the proportion of 1 

 centigram of morphine sulphate for each kilo of body-weight. 



The albumose or peptone was introduced either into the left 

 femoral vein or into the facial vein through a cannula con- 

 nected with a burette. The substance, in the proportion of 

 0.5 gram per kilo of body-weight, was dissolved in 0.7 per cent 

 sodium chloride solution, the volume of the fluid injected 

 ranging from 30 c.c. to 50 c.c. and never exceeding the latter. 

 The fluid was warmed to 40 C. 



To observe the rate at which the blood coagulated, portions 

 of about 5 c.c. each were withdrawn at stated intervals from the 

 right femoral artery through a cannula inserted hi that vessel, 

 the blood being collected in slender test-tubes to observe the 

 time of coagulation. Each time the blood was withdrawn from 

 the artery the first portion passing out was discarded. More- 

 over, the cannula was removed and cleaned after each with- 

 drawal of blood. Blood-pressure was registered at the carotid 

 artery, or in some instances at the left femoral artery, using a 

 Hiirthle spring manometer and a Baltzar kymographion driven 

 at a slow rate. 



Influence on Coagulation of the Blood. The effects of deu- 

 teroalbumose and peptone on the coagulation of the blood 

 were observed in eight experiments on dogs ranging in weight 

 from 5 to 11.5 kilos. In the first experiment the dosage of 

 albumose was 0.33 gram per kilo of body-weight, but in four 

 other experiments the dosage was increased to 0.5 gram per 

 kilo, which proportion was likewise used hi the three experi- 

 ments with peptone. Following are the results obtained : 



FIRST EXPERIMENT. 



Dog, 9 kilos. 3 grams deuteroalbumose in 37 c.c. 0.7 per cent NaCl. 



Injection lasted 2 min. 45 sec. 

 The normal blood coagulated in 3 minutes.* 



Blood withdrawn 3 min. after injection of albumose coagulated in 30 min. 

 p 25 



22 " " " " 27 " 



" 28 " " " " 1-2 hours. 



* The figure given for the coagulation-time of the normal blood is the 

 average of 2-3 determinations. 



