PAPAIN-PROTEOLYSIS. 179 



EIGHTH EXPERIMENT. 



Dog, 5 kilos. Control experiment. 30 c.c. 0.7 per cent NaCl. 



Injection lasted 80 seconds. 

 The normal blood coagulated in 5 minutes. 

 Blood withdrawn 2 min. after injection of salt solution coagulated in 3 min. 



Q (I II <( 4 



Fourteen minutes afterwards 3.0 grams peptone in 30 c.c. 0.7 per cent NaCl 

 were injected. Injection lasted 30 seconds. 



Blood withdrawn 2 min. after injection of peptone coagulated in 10-17 hours. 



" 82 " " " " 3 hrs. 16 min. 



95 u o " 10 " 



From these experiments it is very manifest that both deu- 

 teroalbumose and peptone, as formed from egg-albumin by 

 papain, have a marked effect upon the coagulation of the 

 blood. With the dosage employed, namely, fifty centigrams 

 per kilo of body-weight, coagulation is retarded for periods 

 ranging from thirty minutes to thirty-six hours. Further, 

 some of the experiments seemingly suggest that deuteroal- 

 bumose is somewhat more effective than pure peptone in 

 retarding coagulation. It is likewise noticeable that this 

 retarding effect upon coagulation is much more striking 

 and also more permanent hi some cases than in others, even 

 though the conditions are apparently the same. Thus, in 

 the second and third experiments, in which the dosage of 

 deuteroalbumose per kilo is exactly the same, there is a 

 marked difference in the character of the results, due, how- 

 ever, we believe, to differences in blood-pressure and to con- 

 sequent differences in the rate of elimination through the 

 kidneys. In connection with this last statement it is to be 

 noted that in many of the experiments, with both albumose 

 and peptone, the period of retardation shows a steady decrease 

 (as in the fifth, seventh, and eighth experiments) until event- 

 ually, 50-100 minutes after the injection, the time of coagu- 

 lation approaches somewhere near that of the normal blood. 



What now is to be said regarding the relationship of these 

 bodies in their action on blood-coagulation to the correspond- 



