182 PAPAIN-PROTEOLYSIS. 



a large dose, as observed by Spiro and EUinger with antipep- 

 tone. In conclusion, we see in these results nothing to war- 

 rant the assumption that the two papain products are widely 

 different from ordinary digestive products of a like degree of 

 hydration. They certainly do not differ from the correspond- 

 ing products of pepsin or trypsin digestion more than the 

 latter products differ among themselves. Thus, according 

 to the experiments of Arthus and Huber,* of gelatose, 2 

 grams per kilo of body-weight are required to render the 

 blood of the dog non-coagulable, while of caseose 1.5 grams 

 per kilo are needed ; amounts far larger than are required of 

 an albumose formed from either egg-albumin or blood-fibrin. 

 Indeed, we have observed in a single experiment with pure 

 protogelatose that 5 grams of the substance (dissolved in 

 water) introduced into the facial vein of a dog weighing be- 

 tween three and four kilos, hastened the rate of coagulation. 



Elimination by the Kidneys. As already stated, Neumeister 

 has shown that when ordinary albumoses are introduced into 

 the blood of the dog, they are eliminated in the urine more or 

 less hydrated. The atmidalbumoses, on the contrary, he found 

 were eliminated unchanged. What now is the behavior of 

 the deuteroalbumose formed by papain when similarly injected ? 

 In the second experiment already detailed, in which a dog of 

 6.5 kilos was given 3.25 grams of papain-deuteroalbumose by 

 injection into the facial vein, the bladder (empty at the 

 beginning of the experiment) was found one hour after the 

 injection distended with urine. The fluid, amounting to 150 

 c.c., was removed, filtered, and saturated, boiling hot, with 

 ammonium sulphate. A heavy gummy precipitate resulted, 

 which after being washed with a saturated solution of the am- 

 monium salt, was dissolved in water and tested. It was com- 

 posed of unaltered deuteroalbumose. On testing the filtrate 

 from the latter precipitate, after again boiling with ammonium 

 sulphate to insure the complete removal of the deutero- 



* Arthus and Huber, Action des injections intraveineuses de produits de 

 digestions peptique et tryptique de la gelatine et du case'um sur la coagulation 

 du sang chez le chien. Arch, de physio 1., 1897, 5, viii, p. 857. 



