PAPAIN-PROTEOLYSIS. 183 



albumose, an intense biuret reaction was obtained, thus show- 

 ing plainly the presence of a comparatively large amount of 

 true peptone. In this experiment, therefore, there was marked 

 diuresis, accompanied by a rapid elimination of the deutero- 

 albumose, but most important of all, a large proportion of the 

 eliminated albumose underwent hydration into true peptone 

 during its transit from the blood to the urine. Such a result 

 as this, however, is not always obtained. Thus, hi the third 

 experiment blood-pressure was greatly lowered, and an hour 

 after the injection the bladder contained only a few drops of 

 fluid, with which no distinct reaction for either albumose or 

 peptone could be obtained. In harmony with these two results, 

 the blood in the last experiment drawn 46 minutes after the 

 injection did not coagulate within 18 hours, while in the first 

 experiment, where elimination was comparatively rapid, the 

 blood drawn 49 minutes after the injection coagulated in 38 

 minutes. Further, in the fifth experiment detailed above, 85 

 minutes after injection of the albumose, retardation of blood 

 coagulation was wholly at an end; indeed, coagulation took 

 place more rapidly than prior to the injection. At this time 

 the bladder was found distended with urine, and the latter 

 gave a strong peptone reaction and a fair separation of albu- 

 mose.* In the fourth experiment, there was no marked 

 diuresis, but 76 minutes after the injection the bladder was 

 half full of urine, the latter giving a strong reaction for pep- 

 tone with only a trace of albumose. Thus, the results obtained 

 in this connection certainly warrant the statement that when- 

 ever papain-deuteroalbumose undergoes elimination through 

 the kidneys of the dog, it behaves in the same manner as an 

 ordinary albumose, being transformed in great part into true 

 peptone. It would seem, however, that injection of papain- 

 deuteroalbumose is less liable to produce suspension of the 

 renal secretion than injections of ordinary propeptone.f 



* In a recent preliminary communication (Proceed. Physiol. Soc., Nov. 13, 

 1897), Thompson has likewise reported that Witte's " peptone " and Grosjean's 

 peptone when injected into the jugular vein of dogs may lead to a marked 

 increase in the quantity of urine accompanied by an excretion of part of the 

 albumoses and peptone injected. 



t Compare Grosjean, loc. cit. 



