184 PAPAIN-PROTEOLYSIS. 



With papain-peptone the elimination through the kidneys 

 appeared less marked than with the albumose. Still, in all 

 three experiments the bladder was found, 60-100 minutes after 

 the injection, fairly well filled with urine, and on testing the 

 latter a good biuret reaction for peptone was obtained. In 

 no case was there any separation of an albumose precipitate on 

 saturating the fluid with ammonium sulphate. 



Influence on Blood-pressure. Upon blood-pressure the albu- 

 mose and peptone formed by papain from coagulated egg- 

 albumin have much the same general effect as that produced 

 by ordinary proteoses and peptone. In all of our experiments, 

 in which the pressure was recorded, the dosage of albumose or 

 peptone per kilo was somewhat larger than that ordinarily 

 employed, namely, 0.5 gram. With this dosage, however, 

 there was hi nearly every experiment a marked and rapid fall of 

 pressure lasting for about ten minutes. Moreover, the extent 

 of the fall was seemingly influenced somewhat by the rapidity 

 of the injection, a fact which has been commented upon by 

 Thompson.* Our experiments also incline us to the belief that 

 the character of the result may be modified somewhat by the 

 personality of the animal, independent of the dosage and the 

 duration of the injection. The experiments in this direction, 

 however, were not intended to be exhaustive, but simply to 

 throw light upon the main [problem as to whether the papain 

 products differ radically from ordinary digestive products. In 

 one or two instances the fall of pressure was hardly noticeable, 

 and hi these cases the elimination of the albumose through the 

 urine was quite rapid. The two following experiments may 

 be taken as typical of what was generally observed with 

 deuteroalbumose. 



In a dog weighing 7 kilos, narcotized by chloroform-ether, 

 3.5 grams of deuteroalbumose dissolved in 50 c.c. 0.7 per cent 

 NaCl solution were injected into the right facial vein, the 

 injection lasting 1 min. 15 sec. The blood-pressure was 

 lowered immediately from 160 mm. Hg to about 25 mm. 

 Within four minutes, however, the pressure began to rise 



* Thompson, Journal of Physiology, 1896, xx, p. 460. 



