ON PANCREATIC PROTEOLYSIS. 215 



Experiment 36. Bile salts from pig's bile. Alkaline extract of pig's pancreas. 



Per cent of Bile Salts. Undigested Residue. Fibrin Digested. Relative Proteolytic Action. 



0.4936 gram 50.66 per cent 100.0 



0.6 0.4989 60.11 98.9 



1.0 0.4760 62.60 103.6 



2.0 0.4709 52.91 104.4 



3.0 0.5383 46.17 91.1 



The contrast between the results obtained in the two pre- 

 ceding experiments led to our testing the reaction of the bile 

 salts prepared from pig's bile, and it was found that they 

 were quite strongly acid to litmus. It is thus evident that 

 the marked inhibition of proteolysis observed in Experiment 

 35 was due, in part at least to the increasing acidity of the 

 mixtures. In Experiment 36, on the other hand, the 0.125 

 gram of sodium carbonate present overcame this acidity ex- 

 cept in the mixture containing 3 per cent of the bile salts. 



Experiment 37. Bile salts from pig's bile. Alkaline extract of pig's pancreas. 

 These bile salts were exceedingly acid, hence 0.25 gram Na 2 C0 8 was added 

 to each mixture, thus making the amount of this salt 0.5 per cent in the 

 control. In the presence of 3 per cent of the bile salts, however, the 

 alkalinity was reduced to one-fifth. 



Per cent of Bile Salts. Undigested Residue. Fibrin Digested. Relative Proteolytic Action. 



0.1752 gram 82.48 per cent 100.0 



0.6 0.2045 79.56 96.4 



1.0 0.2142 78.58 952 



2.0 0.2282, 77.18 93.6 



3.0 0.3281 67.19 81.4 



Experiment 38. Bile salts from pig's bile, made neutral before addition. Al- 

 kaline extract of pig's pancreas. Each mixture containing 0.25 per cent 

 Na 2 CO 8 , as usual. 



Per cent of Bile Salts. Undigested Residue. Fibrin Digested. Relative Proteolytic Action, 



0.1846 gram 81.54 per cent 100.0 



0.6 0.2249 77.51 96.0 



1.0 0.2207 77.93 96.6 



2.0 0.2222 77.78 95.3 



aO 0.2324 76.76 94.1 



6.0 0.2543 74.57 91.4 



