216 INFLUENCE OF BILE AND BILE SALTS 



Experiment 39. Bile salts from pig's bile, made neutral before addition. 

 Extract of ox pancreas. 



r>x.,_,oo, h. in^M Undigested Fibrin Relative 



Residue. Digested. Proteolytic Action. 



Neutral + Bile salts 0.5499 gram 45.01 per cent 100.0 



" +1.0% " 0.6467 35.33 78.4 



" +8.0% " 0.6966 30.34 67.4 



" +6.0% " 0.7701 22.99 51.0 



0.25% NajCO, + Bile salts 05748 72.52 161.1 



" " +1-0% " 0.3992 60.08 133.4 



+ 3.0% " 0.5238 47.62 105.7 



+ 5.0% " 0.6760 32.50 72.2 



A critical examination of the foregoing results shows us 

 first that the bile salts from ox bile have no very great influ- 

 ence in either direction upon pancreatic proteolysis. In two 

 experiments (31 and 33) there is some evidence of accelera- 

 tion, while in one experiment retardation is more noticeable. 

 With pure sodium glycocholate (3 per cent) relative proteo- 

 lytic action is reduced from 100 to 87. In Experiment 47, 

 to be quoted later, stimulation of proteolysis is quite marked. 

 In considering these results, however, in their bearing on the 

 influence of fresh bile, it is to be remembered that 3-5 per 

 cent of these bile salts are equivalent to the addition of 40- 

 50 per cent of the original bile. With regard to the apparent 

 difference in action of the several samples of ox bile salts we 

 are inclined to attribute this to variations in the proportion 

 of glycocholate and taurocholate present. Pure glycocholate, 

 other influences'excluded, seems to have a greater inhibitory 

 action than the mixed salts, and possibly the more pronounced 

 retardation seemingly characteristic of the salts from pig's 

 bile is due to the fact that the salts are mainly glycocholates. 

 Still, it is to be observed that the samples of salts from pig's 

 bile vary considerably in the intensity of their action, and 

 this independently of their acidity, for when the latter is neu- 

 tralized the same retarding effect is still produced. Some- 

 what noticeable also is the difference in intensity of action 

 of the neutralized bile salts (from pig's bile) when added to 

 the pancreatic juice of the same species as contrasted with 



