218 INFLUENCE OF BILE AND BILE SALTS 



Fresh pig's bile, very viscid, having a specific gravity of 1036, an acidity 

 of 0.68, and an alkalinity of 1.16, was treated with five volumes of strong 

 alcohol, the precipitate filtered off, and washed with alcohol. The united 

 filtrate and washings were then evaporated for the removal of the alcohol, 

 and the fiuid made up with distilled water to the original volume. The acid- 

 ity was now 0.48, and the alkalinity 0.90. The action of a portion of the 

 original fresh bile and of the bile freed from nucleoalbumin, etc., on proteo- 

 lysis was then tested. 



Experiment 40. With fresh pig's bile. Neutral extract of ox pancreas. 



Per cent of Bile. Undigested Residue. Fibrin Digested. Relative Proteolytic Action. 



0.6777 gram 42.23 per cent 100.0 



0.26 0.6088 30.12 92.6 



0.60 0.6241 37.69 89.0 



1.00 0.7000 80.00 71.0 



2.50 0.7118 28.82 68.2 



6.00 0.6496 36.04 82.9 



10.00 0.7236 27.66 65.4 



Experiment 41. With bile freed from nucleoalbumin. Neutral extract of ox 

 pancreas. 



Per cent of Bile. Undigested Residue. Fibrin Digested, Relative Proteolytic Action. 



0.6446 gram 36.64 per cent 100.0 



0.26 0.6666 84.44 96.9 



0.60 0.6661 38.49 94.2 



1.00 0.7007 29.93 84.2 



2.60 0.7351 26.49 74.5 



6.00 0.7217 27.88 78.3 



10.00 0.6698 34.02 96.7 



position of the various constituents. For this purpose 440 

 c.c. of fresh ox bile, containing 12.4 per cent of solid matter, 

 were evaporated to a very thick syrup on the water-bath and 

 precipitated with absolute alcohol. The small precipitate 

 which resulted was filtered off, washed thoroughly with al- 

 cohol, and then dried over sulphuric acid. It weighed 2.27 

 grams. The alcoholic filtrate was treated with a large volume 

 of ether, the precipitated bile salts filtered off, washed thor- 

 oughly with ether, and dried. The alcohol-ether filtrate was 

 allowed to evaporate, and finally brought to complete dryness 

 on the water-bath. The effect of these three fractions on 

 pancreatic proteolysis was then determined in the usual man- 

 ner. The bile salts and the residue from the alcohol-ether 



