224 



INFLUENCE OF BILE AND BILE SALTS 



Experiment 50. With extract of pig's pancreas. Bile salts from pig's bile 

 made perfectly neutral. Salicylic acid used to combine with the proteids. 



Character of the Fluid. 



/ 



Neutral 



Proteids combined with acid 

 Half saturated with acid (0.063% acid) 

 " " " +0.6% Bile salts 



" +1.0% 

 " " " +2.0% 



" " +3.0% " 



Undigested 

 Residue. 



gnu. 



0.2132 



0.6038 

 0.7659 

 0.8120 

 0.8319 

 0.8900 



Fibrin 

 Digested. 



percent. 

 78.68 



39.62 

 23.41 

 18.80 

 16.81 

 11.00 



Relative 



Proteolytio 



Action. 



100.0 



50.3 

 29.7 

 22.6 

 21.3 

 13.9 



Experiment 51. With extract of ox pancreas. Fresh pig's bile. Salicylic 



acid used to combine with the proteids. 

 Character of the Fluid. 



Neutral 



Proteids combined with acid 

 Half saturated with acid (0.038% acid) 

 " " " + 0.5% Bile 



Undigested Fibrin 

 Residue. Digested. 



grin. 



0.4125 



per cent. 

 68.75 



Relative 

 Proteolytio 



Action. 



100.0 



" + 5.0% " 

 " " " + 10.0% " 



" " " + 20.0% " 



Experiment 52. Neutral extract of ox pancreas. Fresh ox bile. 



In this experiment the proteids of the pancreatic extract were not 

 treated with acid, but sufficient acid was added to the fibrin to saturate it, 

 or half saturate it (as tested by tropaeolin oo), prior to addition of the 

 pancreatic extract. 



Conditions. 



Undigested Fibrin 



Residue. Digested. 



grm. per cent. 



Neutral fibrin 0.3643 63.67 



Fibrin saturated with acid (6 c.c. 0.2% HC1) 0.6755 42.45 



" " " +10% Bile 0.6490 45.10 



" half saturated with acid (2.6 c.c. 0.2 % HC1) 0.4690 63.10 



" + 6% Bile 0.4980 60.20 



Relative 

 Proteplytic 



Action. 



100.0 

 68.3 

 70.9 

 83.5 

 78.9 



In only one of these experiments (Experiment 47) do we 

 see any distinct suggestion of aid to pancreatic proteolysis 

 when bile or bile salts are added to a pancreatic extract con- 

 taining combined acid. Combined acid alone tends to retard 

 proteolysis, and the addition of bile to such mixtures as a rule 



