276 ON THE PATHS OF 



I have repeated the experiment of Asher and Barbara with 

 modifications in two respects. First, a smaller quantity of 

 proteid was fed ; and secondly, the proteid employed was a 

 readily soluble one Witte's "pepton" which previous ex- 

 perience had shown to be satisfactorily utilized by dogs.* A 

 gastric fistula had been made on the 14-kilo animal several 

 months previously. The wound was perfectly healed and the 

 dog in good health, f After forty-eight hours' fasting a glass 

 cannula was tied into the thoracic duct during morphine 

 narcosis (0.16 gram morphine sulphate + 0.016 gram atropine 

 sulphate subcutaneously), a little chloroform-ether being ad- 

 ministered when necessary. The dog remained in narcosis 

 during the experiment. The portion of lymph collected during 

 the first ten minutes after introduction of the cannula was 

 not retained, since owing to the operative procedure the flow 

 of lymph is at first always somewhat more rapid than subse- 

 quently.^ The "hunger" lymph was collected during an 

 hour ; thereupon 55 grams of Witte's " pepton " containing 

 14.0 per cent nitrogen were introduced with 125 c.c. water 

 through the fistula into the stomach. The lymph was then 

 collected in hourly portions during the six succeeding hours. 

 The animal was killed by bleeding and the stomach contents 

 removed and examined. The intestine was found empty ; the 

 appearance of the gastric and intestinal mucosa was normal. 

 The stomach contained 205 c.c. fluid with mucous flocks in 

 suspension. The filtered gastric contents, composed largely 

 of primary proteoses, with a little true peptone, furnished the 

 following data on analysis : 



Total solids .............. 80.23 grams 



Total nitrogen (Kjeldahl) ......... 4.42 " 



Nitrogen in total solids ......... 14.60 per cent 



Total acidity (equivalent to HC1) ...... 0.77 " 



HC1 present. 



* Cf. Mendel and Jackson, Amer. Jour. Physiol., 1898, ii, pp. 10-11. 

 t The form of gastric cannula used has been described in the American 

 Journal of Physiology, 1898, i, p. 191. 



t Cf. Heidenhain, Arch. f. d. ges. Physiol., 1891, xlix, p. 215. 



