CERTAIN DERIVATIVES OF THE PROTEIDS. 303 



strated not only by the marked increase produced in the flow 

 of lymph from the thoracic duct, but likewise by the charac- 

 teristic changes resulting in the composition of the fluid, 

 namely, increase in total solids, the ash-content remaining 

 constant.* In every case where an increased flow of lymph 

 occurred, the fluid grew slightly reddish in color and its clot- 

 ting was markedly delayed, two phenomena characteristic 

 of " lymphagogic " lymph. 



(2) In every case where the lymph was thus diverted from 

 the general circulation the clotting time of the blood was only 

 slightly, if at all affected by the injection, while the coagula- 

 bility of the lymph was delayed. This agrees with the ob- 

 servations of Gley and Pachon, f who announced that the 

 ligature of the lymphatics of the liver prevents the ordinary 

 clot-retarding action of albumose injections in dogs ; and with 

 the observations of Spiro and Ellinger, similar to our own, 

 on dogs with the thoracic duct cannulized.J In other words, 

 the observations lend favor to the view that the anti-clotting 

 substance is formed in the liver and reaches the blood mainly 

 through the lymph. 



(3) A second injection gives rise to a marked renewal of 

 lymph-flow, independent of any action on blood coagulation. 

 The two Experiments XXI and XXIX do not, however, per- 

 mit the immediate conclusion that the lymphagogic action 

 and clot-preventing phenomena are entirely independent, 

 inasmuch as the diversion of the lymph from the circulation 

 may prevent the anti-coagulating substance from reaching 

 the blood. || Since some dogs are naturally immune to albu- 



* Cf. Heidenhain, loc. cit. 



t Gley and Pachon, Comptes rendus de 1'Acad. des Sciences, 1895, p. 383 ; 

 Archives de Physiologic, 1895, p. 711; Comptes rendus de la Soc. de Biologie, 

 1895, p. 741 ; Ibid., 1897. Cf. also Starling, Journal of Physiology, 1895, xix, 

 p. 16 ; Delezenne, Archives de Physiologie, 1896, p. 657. 



} Spiro and Ellinger, Zeitschr. f. physiol. Chem., 1897, xxiii, p. 135. 



Cf. Starling, Journal of Physiology, 1893, xiv, p. 140; also Gley and 

 Pachon, Archives de Physiologie, 1897, p. 861. 



|| Asher and Barbera (Zeitschr. f. Biologic, 1898, xxxvi, p. 154) have put 

 forward the opinion that lymphagogues are substances which stimulate the 

 activity of various organs and especially glands. In agreement with this 



