312 A CHEMICO-PHYSIOLOGICAL STUDY OF 



the hemipeptone from this mixture. A method promising 

 better results is the hydrolytic cleavage of the native proteid 

 with dilute acid, accompanied as it is by the splitting off of 

 the anti groups as antialbumid. While this results in the 

 formation of soluble hemialbumoses and hemipeptone, which 

 can easily be separated, it unfortunately happens that the 

 antialbumid formed also undergoes hydration to some slight 

 degree with consequent formation of some soluble antialbu- 

 moses and antipeptone. These latter bodies, once mixed with 

 the corresponding hemi products, cannot be separated, and 

 hence are an ever-present impurity. Still, this method affords 

 the only way, at present, of obtaining the so-called hemialbu- 

 moses and peptone, and we have had recourse to it as the only 

 available method, understanding, however, that the several 

 products are by no means wholly free from corresponding 

 anti bodies. 



As already stated in the first part of this paper, it seemed 

 very desirable in view of the importance attaching to the 

 physiological action of the various digestive products of the 

 proteids to ascertain as definitely as possible the part played 

 by the several components of the ordinary products of proteo- 

 lytic action. With this end in view we have devoted particular 

 attention to the anti products, and hi order to insure their 

 freedom, so far as possible, from the so-called hemi groups we 

 have made use of antialbumid as the mother substance in the 

 preparation of antialbumoses and antipeptone. 



Hydrolysis and cleavage of coagulated egg albumin with formation 

 of antialbumid, etc. In obtaining sufficient material for our 

 purpose it was necessary to repeat the process several times, 

 but the general line of procedure hi each case was as follows : 

 The whites of twelve dozen eggs were coagulated by pouring 

 them slowly into a large volume of boiling water acidified 

 slightly with acetic acid, the coagulum collected on a cloth 

 filter and washed with large quantities of boiling water. It 

 was then pressed as dry as possible and placed in a large flask 

 with 4 per cent sulphuric acid (360 grams of the moist coagu- 

 lum in 1200 c.c. of the acid). This mixture was then heated 



